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mAbs Against HUMAN Protein

LKB1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:LEY37D
PROTEIN:Serine/Threonine protein kinase 11
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/NP_000446.1
ANTIGEN USED:MBP-LKB1 (full length protein)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2b
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. [provided by RefSeq, Jul 2008].

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Carretero J, Medina PP, Blanco R, Smit L, Tang M, Roncador G, Maestre L, Conde E, López-Ríos F, Clevers HC and Sánchez-Céspedes M. Dysfunctional AMPK activity, signalling through mTOR and survival in response to energetic stress in LKB1-deficient lung cancer. Oncogene. 2007 Mar 8;26(11):1616-25.

REFERENCES

Katia Bouchekioua-Bouzaghou, Coralie Poulard, Juliette Rambaud, Emilie Lavergne, Nader Hussein, Marc Billaud, Thomas Bachelot, Sylvie Chabaud, Sylvie Mader, Guila Dayan, Isabelle Treilleux, Laura Corbo, Muriel Le Romancer. LKB1 when associated with methylatedERα is a marker of bad prognosis in breast cancer. International Journal of Cancer. 15 September 2014.

Shu-Fang Jian, Chang-Chun Hsiao, Shin-Yi Chen, Ching-Chieh Weng, Tzu-Lei Kuo, Deng-Chyang Wu, Wen-Chun Hung, Kuang-Hung Cheng. Utilization of liquid chromatography mass spectrometry analyses to identify LKB1-APC interaction in modulating Wnt/β-catenin pathway of lung cancer cells. Molecular Cancer Research. 1 April 2014.

Wencheng Zhang, Qilong Wang, Ping Song, Ming-Hui Zou. Liver kinase b1 is required for white adipose tissue growth and differentiation. Diabetes. 1 July 2013.
Jinrong Fu, Jing Jin, Robert H Cichewicz, Serena A Hageman, Trevor K Ellis, Lan Xiang, Qi Peng, Mali Jiang, Nicolas Arbez, Katelyn Hotaling, Christopher A Ross, Wenzhen Duan. trans-(-)-ε-Viniferin increases mitochondrial sirtuin 3 (SIRT3), activates AMP-activated protein kinase (AMPK), and protects cells in models of Huntington Disease.The Journal of Biological Chemistry. 13 July 2012.

Ahmed F Salem, Diana Whitaker-Menezes, Zhao Lin, Ubaldo E Martinez-Outschoorn, Herbert B Tanowitz, Mazhar Salim Al-Zoubi, Anthony Howell, Richard G Pestell, Federica Sotgia, Michael P Lisanti. Two-compartment tumor metabolism: autophagy in the tumor microenvironment and oxidative mitochondrial metabolism (OXPHOS) in cancer cells.Cell Cycle. 1 July 2012.

Badal C Roy, Takashi Kohno, Reika Iwakawa, Tetsuo Moriguchi, Tohru Kiyono, Kazuhiro Morishita, Montse Sánchez-Céspedes, Tetsu Akiyama, Jun Yokota. Involvement of LKB1 in epithelial-mesenchymal transition (EMT) of human lung cancer cells. Lung Cancer. 1 November 2010.

Nicolas Humbert, Naveenan Navaratnam, Arnaud Augert, Marco Da Costa, Sébastien Martien, Jing Wang, Dolores Martinez, Corinne Abbadie, David Carling, Yvan de Launoit, Jesús Gil, David Bernard. Regulation of ploidy and senescence by the AMPK-related kinase NUAK1.The EMBO Journal. 20 January 2010.

Xiuyun Hou, Shanqin Xu, Karlene A Maitland-Toolan, Kaori Sato, Bingbing Jiang, Yasuo Ido, Fan Lan, Kenneth Walsh, Michel Wierzbicki, Tony J Verbeuren, Richard A Cohen, Mengwei Zang. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. The Journal of Biological Chemistry. 18 July 2008.

Ping Song, Zhonglin Xie, Yong Wu, Jian Xu, Yunzhou Dong, Ming-Hui Zou. Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells. The Journal of Biological Chemistry. 2 May 2008.

István Vadász, Laura A Dada, Arturo Briva, Humberto E Trejo, Lynn C Welch, Jiwang Chen, Peter T Toth, Emilia Lecuona, Lee A Witters, Paul T Schumacker, Navdeep S Chandel, Werner Seeger, Jacob I Sznajder. AMP-activated protein kinase regulates CO2-induced alveolar epithelial dysfunction in rats and human cells by promoting Na,K-ATPase endocytosis. Journal of Clinical Investigation. 1 February 2008.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
LEY37D neat supernatant /
Paraffin tissue
Immunofluorescence
Enlarge image
LKB1 antibody (LEY37D) in transfected cells
LKB1 staining in transfected HEK293T/LKB1 cells. HEK293T/MNDA transfected cells were used as a negative control.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
LEY37D 0.0002mg/ml / Available
Immunoprecipitation
Enlarge image
LEY37D mAb is able to detect human LKB1 protein by WB.
Lane 1 A543 MUT cell line (100ug) (-)
Lane 2 H23 MUT cell line (100ug) (-)
Lane 3 CALU3 WT cell line (100ug) (+)
Lane 4 H522 WT cell line (100ug) (+)
Lane 5 H1299 WT cell line (100ug) (+)