mAbs Against HUMAN Protein
MALT1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | RON169A |
| PROTEIN: | Mucosa-associated lymphoid tissue lymphoma translocation protein 1 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q9UDY8 |
| ANTIGEN USED: | pCDNA3-MALT1-Flag plasmid vector |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. Involved in the induction of T helper 17 cells (Th17) differentiation. Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation.
Publication describing antibody characterization/validation
Maestre L, Fontan L, Martinez-Climent JA, Garcia JF, Cigudosa JC, Roncador G. Generation of a new monoclonal antibody against MALT1 by genetic immunization. Hybridoma (Larchmt). 2007 Apr; 26 (2):86-91.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | RON169A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
| Immunofluorescence | |||||||||
Enlarge image
- RON169A is able to detect human MALT1 protein in immunocytochemistry
- To confirm that RON169A mAb recognizes human MALT1 protein, immunocytochemistry on frozen cytospin preparations of human MALT1 expressed in HEK293T was performed. Cytospin preparation of myc tagged human Timp3 protein was used as a negative control.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | RON169A | Neat | supernatant | U266 cell line / | 90kDa | 90kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- RON169A mAb is able to detect human MALT1 protein by WB.
- LANES
Lane 1 U266 cell line (100ug) (+)
Lane 2 LP1 cell line (100ug) (+)
Lane 3 SUDHL1 cell line (100ug) (+)
Lane 4 Jurkat cell line (100ug) (+)
Lane 5 Hek-Malt1-flag (100ug) (+)