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mAbs Against HUMAN Protein

MASTL

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:RIPLY74C
PROTEIN:Microtubule associated serine/threonine kinase-like
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/AAH09107.1
ANTIGEN USED:GST-hMASTL recombinant full length protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

MASTL (microtubule associated serine/threonine kinase-like), also known as GW, THC2 or serine/threonine-protein kinase greatwall, is an 879 amino acid serine/threonine kinase regulates mitosis entry and maintenance. Depletion of MASTL delays the G2-to-M phase transition, is linked to a prolonged mitosis and sensitizes cells to mitotic death.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
RIPLY74C 1:15 supernatant Tonsil / mouse Available
Immunofluorescence
Enlarge image
RIPLY74C mAb in transfected cells
Cytospin preparations of transfected HEK293T/v5-hMASTL and HEK293T/V5-mMASTL cells using antibody RIPLY74C. Anti-V5 antibody was used as positive control.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
RIPLY74C 1:50 neat supernatant Raji cell line / 97kDa 120kDa Available
Immunoprecipitation
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Western Blotting of MAST (RIPLY74C) using different human cell lines.
Lane 1 Hek-hMASTL transfected cells (20ug) (+)
Lane 2 Hek-mMASTL transfected cells (20ug) (+)
Lane 3 Hek-FLT4 transfected cells (20ug) (-)
Lane 4 Raji cell line (100ug)(+)
Lane 5 Ramos cell line (100ug)(+)
Lane 6 Dana cell line (100ug)(+)
Lane 7 MT-2 cell line (100ug)(+)
Lane 8 human tonsil (100ug)(-)
Lane 9 Human testis (100ug)(-)
Enlarge image
Western Blotting of MASTL (RIPLY74C) using different cell lines
Lane 1 293T cell mock transfected (40ug)
Lane 2 293T cells V5-mMASTL (40ug)
Lane 3 HeLa cells shCtrl transfected (40ug)
Lane 4 HeLa cells shMASTL transfected (40ug)