mAbs Against HUMAN Protein
NKX2.3
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti human |
| CLONE NAME: | 5GAL454C |
| PROTEIN: | Homeobox protein Nkx-2.3 (Homeobox protein NK-2 homolog C) |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/Q8TAU0 |
| ANTIGEN USED: | hNKX-2.3-GST recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
This gene encodes a homeodomain-containing transcription factor. The encoded protein is a member of the NKX family of homeodomain transcription factors. Studies of similar proteins in mouse and rat have indicated a potential role in cellular differentiation.[provided by RefSeq, Mar 2010]
References
Homeobox NKX2-3 promotes marginal-zone lymphomagenesis by activating B-cell receptor signalling and shaping lymphocyte dynamics. Eloy F. Robles, Maria Mena-Varas, Laura Barrio, Sara V. Merino-Cortes, Péter Balogh, Ming-Qing Du, Takashi Akasaka, Anton Parker, Sergio Roa, Carlos Panizo, Idoia Martin-Guerrero, Reiner Siebert, Victor Segura, Xabier Agirre, Laura Macri-Pellizeri, Beatriz Aldaz, Amaia Vilas-Zornoza, Shaowei Zhang, Sarah Moody, Maria Jose Calasanz, Thomas Tousseyn, Cyril Broccardo, Pierre Brousset, Elena Campos-Sanchez, Cesar Cobaleda, Isidro Sanchez-Garcia, Jose Luis Fernandez-Luna, Ricardo Garcia-Muñoz, Esther Pena, Beatriz Bellosillo, Antonio Salar, Maria Joao Baptista, Jesús Maria Hernandez-Rivas, Marcos Gonzalez, Maria Jose Terol, Joan Climent, Antonio Ferrandez, Xavier Sagaert, Ari M. Melnick, Felipe Prosper, David G. Oscier, Yolanda R. Carrasco, Martin J. S. Dyer, and Jose A. Martinez-Climent.
Nat Commun. 2016; 7.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 5GAL454C | 1:10 | supernatant | Tris-EDTA | Novolink | Spleen / | nuclear | Available | |
| Immunofluorescence | |||||||||
Enlarge image
- Cytospin slides of transfected HEK cells.
- The transfected HEK cell line with NKX2.3 showed specific labelling with 5GAL454C antibody. HEK-LILRB3 transfected cells were used as negative control. Anti-myc antibody was used as positive control.
Enlarge image
- Immunostaining of 5GAL454C antibody on human paraffin tissues.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 5GAL454C | Neat supernatant | Human spleen / | 38kDa | 38kDa | Available | ||
| Immunoprecipitation | ||||||||
Enlarge image
- WB of 5GAL454C mAb in human cell lines.
- Lane 1 HEK-NKX2.3-MYC transfected cells (+)
Lane 2 HEK-NKX2.2-MYC transfected cells (-)
Lane 3 Human spleen (+)
Lane 4 Human spleen (+)
Lane 5 Human K1718 splenic lymphoma cell line (-)
Anti-Vinculin was used as a loading control.