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mAbs Against HUMAN Protein

Nucleophosmin

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:NA24
PROTEIN:Nucleophosmin
PROTEIN WEB:https://www.omim.org/entry/164040
ANTIGEN USED:Nucleophosmin recombinant protein
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:ChIP IHC IF IP WB

DESCRIPTION

Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successul chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a a suppressor of centrosome duplication.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Blood. 1999 Jan 15;93(2):632-42.
Detection of normal and chimeric nucleophosmin in human cells.
Cordell JL1, Pulford KA, Bigerna B, Roncador G, Banham A, Colombo E, Pelicci PG, Mason DY, Falini B.
PMID: 9885226
https://www.ncbi.nlm.nih.gov/pubmed/9885226

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
NA24 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
NA24 Tonsil
Tonsil stained with neat supernatant from NA24 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
NA24 Tonsil
Tonsil stained with neat supernatant from bcl-2/124 hybridoma, detection using Dako Envision kit, 20x power image