mAbs Against HUMAN Protein
|CONTACT INFORMATION:||University of Oxford marketed by Ximbio.com|
|TYPE:||mouse anti human|
|ANTIGEN USED:||Nucleophosmin recombinant protein|
|PREPARATION AND STORAGE:||Aliquot and store at 4C. Do not freeze|
|APP RECOMMENDED:||ChIP IHC IF IP WB|
Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. NPM also localises between the paired centrioles of the centrosome and dissociates upon the phosphorylation of on Thr199 by CDK2/cyclin E prior to the initiation of centrosome duplication, an essential process for successul chromosome segregation during mitosis. The down-regulation of this protein results in the abnormal amplification of centrosomes which suggests that this protein may act as a a suppressor of centrosome duplication.
Publication describing antibody characterization/validation
Blood. 1999 Jan 15;93(2):632-42.
Detection of normal and chimeric nucleophosmin in human cells.
Cordell JL1, Pulford KA, Bigerna B, Roncador G, Banham A, Colombo E, Pelicci PG, Mason DY, Falini B.
|IHC Techniques||Clone||Dilution||Antibody concentration||Antigen retrieval method||Visualization kit||+/- control||Protein localization||Positivity in other species||Protocol|
|Frozen tissue and cytospins|
|NA24||neat||supernatant||Tris EDTA||Dako Envision||Tonsil /|
- NA24 Tonsil
- Tonsil stained with neat supernatant from NA24 hybridoma, detection using Dako Envision kit, 4x power image
- NA24 Tonsil
- Tonsil stained with neat supernatant from bcl-2/124 hybridoma, detection using Dako Envision kit, 20x power image