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mAbs Against HUMAN Protein

PDGFB

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti human
CLONE NAME:326A
PROTEIN:Platelet derived growth factor, B chain
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/NP_002599.1
ANTIGEN USED:His-GST-PDGFB recombinant full length protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2b
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are asociated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013].

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
326A 1:20 Purified Tonsil / Available
Immunofluorescence
Enlarge image
326A mAb in transfected cells
Cytospin preparations of transfected HEK293T/v5-hPGFB and HEK293T/GFP-hPDGFA cells using antibody 326A. Anti-V5 anti-GFP antibodies were used to confirm the efficiency of the transfections.
Enlarge image
326A mAb staining in human paraffin tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Inconclusive Result obtained is not completely satisfactory. More testing are needed before using these reagents in this application
326A Neat supernatant / 27kDa 27kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of 326A using different cell extracts.
Lane 1 Hek-V5-PDGFB (0.1ug) (+)
Lane 2 Hek-LMO2 (100ug) (-)
Lane 3 Ocily3 (100ug) (-)
Lane 4 Ocily19 (100ug) (+)
Lane 5 DOHH2 (100ug) (-)
Lane 6 Toledo (100ug) (+)
Lane 7 Raji (100ug) (-)
Lane 8 Ramos (100ug) (-)
Lane 9 Mutu1 (100ug) (-)
Lane 10 U251 (100ug) (+)
Lane 11 U87MG (100ug) (+)
Lane 12 SN75B (100ug) (+)
Lane 13 A431 (100ug) (+)
Lane 14 YT (100ug) (+)
Lane 15 Jurkat (100ug) (+)
Lane 16 Karpas (100ug) (-)