mAbs Against HUMAN Protein
|CONTACT INFORMATION:||Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas|
|PROTEIN:||Serine/threonine-protein kinase PLK1|
|ANTIGEN USED:||HIS-hPlk1 full-length protein|
|FUSION PARTNER:||NS1/Ag4-1 (NS1) cells|
|SPECIES REACTIVITY:||human and mouse|
|PREPARATION AND STORAGE:||Aliquot and store at 4C. Do not freeze|
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains.
Wachowicz P, Fernández-Miranda G, Marugán C, Escobar B, de Cárcer G. Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies. Bioessays. 2016 Jul;38 Suppl 1:S96-S106. doi: 10.1002/bies.201670908. PubMed PMID: 27417127.
Trakala M, Partida D, Salazar-Roa M, Maroto M, Wachowicz P, de Cárcer G, Malumbres M. Activation of the endomitotic spindle assembly checkpoint and thrombocytopenia in Plk1-deficient mice. Blood. 2015 Oct 1;126(14):1707-14. doi: 10.1182/blood-2015-03-634402. PubMed PMID: 26185128.
|IHC Techniques||Clone||Dilution||Antibody concentration||Antigen retrieval method||Visualization kit||+/- control||Protein localization||Positivity in other species||Protocol|
|Frozen tissue and cytospins|
Recommended Result obtained is satisfactory. The reagent can be use in this application
- POE125A is able to detect human and mouse Plk1 protein in immunocytochemistry
- To confirm that POE125A mAb recognizes human and mouse PLK1 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged hPLK1 and mPLK1 expressed in HEK293T cell line was performed