EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network homepage


mAbs Against HUMAN Protein


STATUS:Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
TYPE:Rat monoclonal
PROTEIN:Serine/threonine-protein kinase PLK1
ANTIGEN USED:HIS-hPlk1 full-length protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze


Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains.


Wachowicz P, Fernández-Miranda G, Marugán C, Escobar B, de Cárcer G. Genetic depletion of Polo-like kinase 1 leads to embryonic lethality due to mitotic aberrancies. Bioessays. 2016 Jul;38 Suppl 1:S96-S106. doi: 10.1002/bies.201670908. PubMed PMID: 27417127.

Trakala M, Partida D, Salazar-Roa M, Maroto M, Wachowicz P, de Cárcer G, Malumbres M. Activation of the endomitotic spindle assembly checkpoint and thrombocytopenia in Plk1-deficient mice. Blood. 2015 Oct 1;126(14):1707-14. doi: 10.1182/blood-2015-03-634402. PubMed PMID: 26185128.


IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
POE125A Neat supernatant /
Paraffin tissue
Enlarge image
POE125A is able to detect human and mouse Plk1 protein in immunocytochemistry
To confirm that POE125A mAb recognizes human and mouse PLK1 protein, immunocytochemistry on frozen cytospin preparations of GFP-tagged hPLK1 and mPLK1 expressed in HEK293T cell line was performed