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mAbs Against HUMAN Protein

Proliferation marker

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:JC1
PROTEIN:Proliferation marker
ANTIGEN USED:Pokeweed-stimulated lymphocyte
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC IF WB

DESCRIPTION

JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Clin Pathol. 1992 Oct;45(10):860-5.
Monoclonal antibody JC1: new reagent for studying cell proliferation.
Garrido MC1, Cordell JL, Becker MH, Key G, Gerdes J, Jones M, Gatter KC, Mason DY.
PMID: 1430255
https://www.ncbi.nlm.nih.gov/pubmed/1430255

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
JC1 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
JC1 Tonsil
Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
JC1 Tonsil
Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
JC1 Tonsil
Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 20x power image