mAbs Against HUMAN Protein
Proliferation marker
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | JC1 |
| PROTEIN: | Proliferation marker |
| ANTIGEN USED: | Pokeweed-stimulated lymphocyte |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC IF WB |
Description
JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Publication describing antibody characterization/validation
J Clin Pathol. 1992 Oct;45(10):860-5.
Monoclonal antibody JC1: new reagent for studying cell proliferation.
Garrido MC1, Cordell JL, Becker MH, Key G, Gerdes J, Jones M, Gatter KC, Mason DY.
PMID: 1430255
https://www.ncbi.nlm.nih.gov/pubmed/1430255
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| JC1 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- JC1 Tonsil
- Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- JC1 Tonsil
- Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- JC1 Tonsil
- Tonsil stained with neat supernatant from JC1 hybridoma, detection using Dako Envision kit, 20x power image