mAbs Against HUMAN Protein
Spectrin
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | RG/26 |
| PROTEIN: | Spectrin |
| ANTIGEN USED: | Red cell membranes |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC IF IP WB |
Description
Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.
References
Vox Sang. 2014 Jan;106(1):45-54. doi: 10.1111/vox.12074. Epub 2013 Aug 2.
Impact of constant storage temperatures and multiple warming cycles on the quality of stored red blood cells.
Wagner T1, Pabst MA, Leitinger G, Reiter U, Kozma N, Lanzer G, Huppertz B.
PMID: 23909508
https://www.ncbi.nlm.nih.gov/pubmed/23909508
Asian Pac J Allergy Immunol. 2008 Mar;26(1):37-45.
Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells.
Sachanonta N1, Medana IM, Roberts R, Jones M, Day NP, White NJ, Ferguson DJ, Turner GD, Pongponratn E.
PMID: 18595528
https://www.ncbi.nlm.nih.gov/pubmed/18595528
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| RG/26 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- RG/26 Tonsil
- Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- RG/26 Tonsil
- Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- RG/26 Tonsil
- Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 10x power image