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mAbs Against HUMAN Protein

Spectrin

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:RG/26
PROTEIN:Spectrin
ANTIGEN USED:Red cell membranes
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC IF IP WB

DESCRIPTION

Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

REFERENCES

Vox Sang. 2014 Jan;106(1):45-54. doi: 10.1111/vox.12074. Epub 2013 Aug 2.
Impact of constant storage temperatures and multiple warming cycles on the quality of stored red blood cells.
Wagner T1, Pabst MA, Leitinger G, Reiter U, Kozma N, Lanzer G, Huppertz B.
PMID: 23909508
https://www.ncbi.nlm.nih.gov/pubmed/23909508

Asian Pac J Allergy Immunol. 2008 Mar;26(1):37-45.
Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells.
Sachanonta N1, Medana IM, Roberts R, Jones M, Day NP, White NJ, Ferguson DJ, Turner GD, Pongponratn E.
PMID: 18595528
https://www.ncbi.nlm.nih.gov/pubmed/18595528

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
RG/26 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
RG/26 Tonsil
Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
RG/26 Tonsil
Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
RG/26 Tonsil
Tonsil stained with neat supernatant from RG/26 hybridoma, detection using Dako Envision kit, 10x power image