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mAbs Against HUMAN Protein


STATUS:Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
TYPE:Rat monoclonal
PROTEIN:Methylcytosine dioxygenase TET2
ANTIGEN USED:TET2-HIS fragment (1388-2002aa) recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1 lambda
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze


Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.


IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
CADA359A Neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
CADA359A 1:5 supernatant Tris-EDTA Novolink Tonsil / nuclear Available
Enlarge image
CADA359A mAb is able to detect human TET2 protein in immunocytochemistry.
To confirm that CADA359A mAb recognizes human TET2 protein, immunocytochemistry on frozen cytospins preparations of HA, GFP and FLAG-tagged human TET1, TET2 and TET3 expressed in HEK293 was performed. Anti-HA, GFP and FLAG antibodies were used as positive control. Cytospin preparation of FLAG-tagged human SYNCYTIN1 was used as a negative control.
Enlarge image
CADA359A antibody can be used to detect TET2 protein in human paraffin tissues.