mAbs Against HUMAN Protein
TET2
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | CADA359A |
| PROTEIN: | Methylcytosine dioxygenase TET2 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q6N021 |
| ANTIGEN USED: | TET2-HIS fragment (1388-2002aa) recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 lambda |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CADA359A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CADA359A | 1:5 | supernatant | Tris-EDTA | Novolink | Tonsil / | nuclear | Available | |
| Immunofluorescence | |||||||||
Enlarge image
- CADA359A mAb is able to detect human TET2 protein in immunocytochemistry.
- To confirm that CADA359A mAb recognizes human TET2 protein, immunocytochemistry on frozen cytospins preparations of HA, GFP and FLAG-tagged human TET1, TET2 and TET3 expressed in HEK293 was performed. Anti-HA, GFP and FLAG antibodies were used as positive control. Cytospin preparation of FLAG-tagged human SYNCYTIN1 was used as a negative control.
Enlarge image
- CADA359A antibody can be used to detect TET2 protein in human paraffin tissues.