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Cytospin preparation technique

Protocol created by Lorena Maestre - Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas

Cytospin preparations

A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined. This technique has a wide variety of applications including cytology, histology and hematology.

Equipment, reagents and cell preparations


Figure 1. Thermo Scientific™ Cytospin™ 4 Cytocentrifuge.
Preparation of a slide in cytospin holder: Slide clip, slide, filter card, cytofunnel
Figure 2. Preparation of a slide in cytospin holder for running in a ™ Cytospin™ 4 centrifuge. Picture taken from:
Cytospin and immunoperoxidase
Figure 3. a) Cytospin preparation stained with haematoxylin to show the morphology of NPM-ALK COS transfectants. b) Immunoperoxidase labeling of NPM-ALK COS transfected cells stained with anti-ALK antibody.