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Protocols

Immunofluorescence staining

Protocol created by Lorena Maestre - Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas

Preparation of the slides

  1. Frozen sections
    • Cut 4-8 um thick cryostat sections and mount on superfrost plus slides.
    • Air dry de slides overnight.
    • Fix in acetone for 10 min and store at -20º or -80º until needed.
    • Before staining, warm slides at room temperature.
  2. Paraffin sections
    • Deparaffinize sections in xylene, 2x5min.
    • Hydrate with 100% ethanol, 2x3min.
    • Hydrate with 95% ethanol, 1min.
    • Rinse in distilled water.

Pre-treatment of tissue sections

Procedure

Appendix I