mAbs Against MOUSE Protein
BCL7A
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | 15C |
| PROTEIN: | Bcl7a human full length |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/omim/601406 |
| ANTIGEN USED: | His-GST-Bcl7a recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-paraffin, IF |
| APP NO RECOMMENDED: | WB, IHQ-frozen |
| APP NO TESTED: | IP, Flow cytometry |
Description
This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma.
Publication describing antibody characterization/validation
BCL7A protein expression in normal and malignant lymphoid tissues. Ramos-Medina R, Montes-Moreno S, Maestre L, Cañamero M, Rodríguez-Pinilla M, Martínez-Torrecuadrada J, Piris MÁ, Majid A, Dyer MJ, Pulford K, Roncador G. Br J Haematol. 2013 Jan;160(1):106-9.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 15C | supernatant | Discovery Xt CC1 OmniMap | Ventana | lymph node / | nuclear | human | ||
| Immunofluorescence | |||||||||
Enlarge image
- Anti-BCL7A antibody in mouse paraffin tissues