mAbs Against MOUSE Protein
beta-galactosidase
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | CNB-CSIC Protein Tools Unit, Centro Nacional de Biotecnología/CSIC |
| STATUS: | Validated |
| TYPE: | rat anti E.coli |
| CLONE NAME: | 3A9A |
| PROTEIN: | Beta-galactosidase |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/18073591 |
| ANTIGEN USED: | Beta-gal-monomer-KLH |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2b |
| SPECIES REACTIVITY: | N/A |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-paraffin, WB, IF |
| APP NO RECOMMENDED: | IHQ-frozen |
| APP NO TESTED: | IP, Flow cytometry |
Description
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 3A9A | 1:5 | supernatant | Discovery Xt CC1 OmniMap | Ventana | beta-gal expressing tissue / | |||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 3A9A | neat | supernatant | beta-gal expressing tissue / | Available | ||||
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- Beta-gal antibody (3A9A) in transfected cells
- Validation of 3A9A monoclonal antibody in Hek-V5-Beta-gal transfected cells. Anti Beta-gal (Cappel) was used as positive control. Hek-v5-A-myb transfected cells were used as negative control.
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- Anti-beta-gal (Clone 3A9A) immunohistochemistry in mouse paraffin tissues.
- In the present mouse model, Beta galactosidase expression is restricted to endothelial blood vessels.
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- Beta-Gal expression in mouse paraffin tissue.
- Rat monoclonal 3A9A can be used to detect Beta-Gal over expression on mouse paraffin sections. Intestine of a mouse expressing β-Gal driven by the Rosa26 promoter and wild type mouse intestine.
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- Expression of Beta-gal (3A9A) antigen by mouse pancreatic cells.
- Pancreas of a knockin mouse line expressing Beta-Gal driven by the K-Ras promoter (β-Gal expression can be observed in a small number of pancreatic cells).
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- Beta gal expression in mouse adenoma.
- Beta gal (Red) and KI-67 (green) in mouse kras driven adenoma.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 3A9A | neat | supernatant | / | 110 | Available | ||
| Immunoprecipitation | ||||||||
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- Biochemical characterization of anti-Beta-gal (3A9A) monoclonal antibody
- Western blot analysis of Beta-gal expression in total protein extracts from transfected cells and transgenic mice expressing beta-gal protein.
Lane 1 Hek-v5-beta-gal transfected cells (20ug) (+)
Lane 2 Hek-v5-BTLA transfected cells (20ug) (-)
Lane 3 Mouse kidney extract expressing beta-gal (100ug) (+)
Lane 4 Mouse kidney extract (100ug) (-)
Lane 5 Mouse brain extract expressing beta-gal (100ug) (+)
Lane 6 Mouse brain extract (100ug) (-)
Lane 7 Mouse pancreas extract expressing beta-gal (100ug) (+)
Lane 8 Mouse pancreas extract (100ug) (-)
Lane 9 Mouse lung extract expressing beta-gal (100ug) (+)
Lane 10 Mouse lung extract (100ug) (-)