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mAbs Against MOUSE Protein

beta-galactosidase

Antibody Validated
CONTACT INFORMATION:CNB-CSIC Protein Tools Unit, Centro Nacional de Biotecnología/CSIC
STATUS:Validated
TYPE:rat anti E.coli
CLONE NAME:3A9A
PROTEIN:Beta-galactosidase
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/18073591
ANTIGEN USED:Beta-gal-monomer-KLH
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2b
SPECIES REACTIVITY:N/A
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:IHQ-paraffin, WB, IF
APP NO RECOMMENDED:IHQ-frozen
APP NO TESTED:IP, Flow cytometry

DESCRIPTION

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
3A9A 1:5 supernatant Discovery Xt CC1 OmniMap Ventana beta-gal expressing tissue /
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
3A9A neat supernatant beta-gal expressing tissue / Available
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Beta-gal antibody (3A9A) in transfected cells
Validation of 3A9A monoclonal antibody in Hek-V5-Beta-gal transfected cells. Anti Beta-gal (Cappel) was used as positive control. Hek-v5-A-myb transfected cells were used as negative control.
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Anti-beta-gal (Clone 3A9A) immunohistochemistry in mouse paraffin tissues.
In the present mouse model, Beta galactosidase expression is restricted to endothelial blood vessels.
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Beta-Gal expression in mouse paraffin tissue.
Rat monoclonal 3A9A can be used to detect Beta-Gal over expression on mouse paraffin sections. Intestine of a mouse expressing β-Gal driven by the Rosa26 promoter and wild type mouse intestine.
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Expression of Beta-gal (3A9A) antigen by mouse pancreatic cells.
Pancreas of a knockin mouse line expressing Beta-Gal driven by the K-Ras promoter (β-Gal expression can be observed in a small number of pancreatic cells).
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Beta gal expression in mouse adenoma.
Beta gal (Red) and KI-67 (green) in mouse kras driven adenoma.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
3A9A neat supernatant / 110 Available
Immunoprecipitation
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Biochemical characterization of anti-Beta-gal (3A9A) monoclonal antibody
Western blot analysis of Beta-gal expression in total protein extracts from transfected cells and transgenic mice expressing beta-gal protein.
Lane 1 Hek-v5-beta-gal transfected cells (20ug) (+)
Lane 2 Hek-v5-BTLA transfected cells (20ug) (-)
Lane 3 Mouse kidney extract expressing beta-gal (100ug) (+)
Lane 4 Mouse kidney extract (100ug) (-)
Lane 5 Mouse brain extract expressing beta-gal (100ug) (+)
Lane 6 Mouse brain extract (100ug) (-)
Lane 7 Mouse pancreas extract expressing beta-gal (100ug) (+)
Lane 8 Mouse pancreas extract (100ug) (-)
Lane 9 Mouse lung extract expressing beta-gal (100ug) (+)
Lane 10 Mouse lung extract (100ug) (-)