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Leonor Kremer

Name of the laboratory

CNB-CSIC Protein Tools Unit
CNB-CSIC Protein Tools Unit
Centro Nacional de Biotecnología (CNB)
Spanish National Research Council (CSIC)
Darwin 3, Campus Cantoblanco
28049 Madrid, Spain
(+34) 915 854 614

Members of the laboratory

Head of the Unit
Leonor Kremer
Leonor Kremer (PhD) lkremer@cnb.csic.es
Lab Members
Mónica García-Gallo
Mónica García-Gallo (PhD) mggallo@cnb.csic.es
María Teresa Martín
María Teresa Martín (PhD) mtmartin@cnb.csic.es
Mercedes Llorente
Mercedes Llorente (BSc) llorente@cnb.csic.es
Laura Martín
Laura Martín (BSc) lmartin@cnb.csic.es
Carolina Sánchez
Carolina Sánchez cschez@cnb.csic.es
Lucio Gómez
Lucio Gómez lgomez@cnb.csic.es

Laboratory activity

The CNB-CSIC Protein Tools Unit focuses on the design, creation and use of proteins as specific molecular tools. The Unit generates monoclonal antibodies (mAb), performs studies of the immune response, develops specific immunoassays and analyzes biomolecular interactions using a Biacore 3000 surface plasmon resonance biosensor. It works in a horizontal manner, participating in research projects or on contract for specific goals. The activities, individually or as part of a project, are based on proposals from research groups within the CNB and from private or public research organizations and/or companies.

Our main goal is to generate mAb not available commercially. These mAb, made for research purposes, can also be used for patent and commercial applications; we also help basic research groups with technology transfer from research. In its first two years of activity (2007-2008), the Unit generated nearly 100 mAb against more than 30 antigens, for use in basic and pre-clinical research, drug discovery, and diagnostics. We currently produce mAb to chromatin-associated proteins (Dido, ScoHET), kinases (P38, PI3K, DGK, GSK3, PKD), receptors (Dectin-1, Kidins220), viral antigens (TEGV, SARS) and other human pathogens (Candida famata, Anisakis spp).

Research activities

Monoclonal antibody development and characterization

We have developed mAb for structure-function studies, antigen mapping, neutralization, quantitative assays, pharmacokinetics, and affinity determination of enzymes (alkaline phosphatase, RNAse), nucleases (CEL I), transcription factors (DREAM), hormones (LH, FSH, TSH, hCG, hGH, hGH antagonist B2036, Pegvisomant), growth factors (IGF-1, IGF-2, EGF), growth factor-binding proteins (IGFBP-1, IGFBP-3, GHBP), cytoskeletal proteins (4.1R, WIP), receptors (IGF-1R, EGFR, GHR, CCR4, CCR6, CCR8, CCR9), integral membrane proteins (MAL, BENE), plasma and coagulation proteins (albumin, immunoglobulins, Factor VIII), chemokines (CCL1, CCL11, CCL20), virus (Phi 29, torovirus) and tumor antigens (FBP). These mAb have been used by various international research groups and have been described in more than 200 scientific publications.

Production and characterization of antibodies to murine chemokine receptors using rats and knockout mice

We have generated a panel of poly- and monoclonal antibodies to chemokine receptors for studies of receptor-ligand interactions and for phenotyping mouse models of human inflammatory diseases. Some anti-mouse CCR4 and CCR8 mAb were produced by immunization of rats and knockout mice. Several of these mAb block ligand-receptor binding. One of them has been used by various research groups to inhibit CCR8 function in distinct mouse models of inflammatory disease and has been licensed to a company for worldwide commercialization.

Production and characterization of mAb to human chemokine receptors

We have generated a panel of mouse mAb to human chemokine receptors CCR6 and CCR9, one of which recognizes hCCR6 in flow cytometry and has been licensed for commercialization.

Production of poly- and monoclonal antibodies to cell signaling molecules

Our current research focuses on phosphospecific antibodies against kinases, including glycogen synthase kinase 3 (GSK3), protein kinase D (PKD), class I(A) phosphoinositide 3-kinase (PI3K), SADB Ser/Thr kinase, diacylglycerol kinase (DGK) and a specific epitope in the p38 MAP kinase (MAPK) alternative pathway.

Techniques available

Publications (2012-present)

Patents

Members