mAbs Against MOUSE Protein
Estrogen Receptor Alpha
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | CRET94D |
| PROTEIN: | Estrogen Receptor Alpha (ESR1) |
| PROTEIN WEB: | http://www.hprd.org/alternate?hprd_id=00589&isoform_id=00589_4&isoform_name=Isoform_1 |
| ANTIGEN USED: | MBP-CRE-ERT recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cell |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
The oestrogen receptor alpha (ESR1) is a transcription factor that potentiates the response to diverse stimuli, including oestrogen and growth factors, in various tissue types. Its recruitment to the DNA is directly regulated by the chromatin landscape, inclusive of chromatin compaction and epigenetic modifications.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CRET94D | undiluted | supernatant | OmniMap Mild CC1 | Ventana | Uterus / | human | ||
| Immunofluorescence | |||||||||
Enlarge image
- Estrogen receptor expression in mouse paraffin ovary.
- The immunohistochemistry in mouse ovaries embedded in paraffin (wt, ER alpha ko and ER beta ko) confirms the specificity of the antibody.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | CRET94D | NEAT | Supernatant | uterus / | 66kDa | 66kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- Western blotting of mice tissue extract
- Lane 1 Mouse uterus wt (100ug)
Lane 2 Mouse uterus KO ERA (100ug)
Lane 3 Mouse uterus KO ERB (100ug)