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mAbs Against MOUSE Protein

Estrogen Receptor Alpha

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti mouse
CLONE NAME:CRET94D
PROTEIN:Estrogen Receptor Alpha (ESR1)
PROTEIN WEB:http://www.hprd.org/alternate?hprd_id=00589&isoform_id=00589_4&isoform_name=Isoform_1
ANTIGEN USED:MBP-CRE-ERT recombinant protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG1
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

The oestrogen receptor alpha (ESR1) is a transcription factor that potentiates the response to diverse stimuli, including oestrogen and growth factors, in various tissue types. Its recruitment to the DNA is directly regulated by the chromatin landscape, inclusive of chromatin compaction and epigenetic modifications.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
CRET94D undiluted supernatant OmniMap Mild CC1 Ventana Uterus / human
Immunofluorescence
Enlarge image
Estrogen receptor expression in mouse paraffin ovary.
The immunohistochemistry in mouse ovaries embedded in paraffin (wt, ER alpha ko and ER beta ko) confirms the specificity of the antibody.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
CRET94D NEAT Supernatant uterus / 66kDa 66kDa Available
Immunoprecipitation
Enlarge image
Western blotting of mice tissue extract
Lane 1 Mouse uterus wt (100ug)
Lane 2 Mouse uterus KO ERA (100ug)
Lane 3 Mouse uterus KO ERB (100ug)