mAbs Against MOUSE Protein
FOXP3
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | 221D |
| PROTEIN: | Human full length FOXP3 |
| ANTIGEN USED: | GST-FOXP3 recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human, mouse, cat, dog, horse, rabbit, alpaca and pig. |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-paraffin, IHQ-frozen, WB, Flow cytometry, IF |
| APP NO TESTED: | IP |
Description
FOXP3 is a forkhead-family transcription factor that negatively regulates T cell function. Mice carrying a loss-of-function mutation in FoxP3 present with fatal autoimmune-like disease caused by hyperresponsive CD4(+) T cells. Mice that overexpress scurfin possess fewer mature T cells with reduced functional capabilities compared with normal littermate control mice. FOXP3 is critical for normal CD4+ T cell function and for the successful coordination of a normal response to immunological challenge in vivo.
Publication describing antibody characterization/validation
Roncador G, Brown PJ, Maestre L, Hue S, Martínez-Torrecuadrada JL, Ling KL, Pratap S, Toms C, Fox BC, Cerundolo V, Powrie F, Banham AH. Analysis of FOXP3 protein expression in human CD4(+)CD25(+) regulatory T cells at the single-cell level. Eur J Immunol 2005 35:1681-1691. http://www.ncbi.nlm.nih.gov/pubmed/15902688
Banham AH, Lyne L, Scase TJ, Blacklaws BA. Monoclonal antibodies raised to the human FOXP3 protein can be used effectively for detecting Foxp3(+) T cells in other mammalian species. Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):376-81. http://www.sciencedirect.com/science/article/pii/S0165242708007083
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 221D | neat | supernatant | OmniMap Mild CC1 | Ventana | lymph node / | nuclear | human | |
| Immunofluorescence | |||||||||
Enlarge image
- Anti-FOXP3 221D in mouse paraffin sections.