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mAbs Against MOUSE Protein

FRA2

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:Rat anti mouse
CLONE NAME:REY146C
PROTEIN:Fos like antigen 2
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/nucleotide/229577213?report=genbank&log$=nuclalign&blast_rank=1&RID=ZNM5H8A201R
ANTIGEN USED:HIS-mFRA2 (178-326aa) recombinant protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

REFERENCES

Wurm S, Zhang J, Guinea-Viniegra J, García F, Muñoz J, Bakiri L, Ezhkova E, Wagner EF. Terminal epidermal differentiation is regulated by the interaction of Fra-2/AP-1 with Ezh2 and ERK1/2. Genes dev.2015 Jan 15;29 (2):144-56.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
REY146C Neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
REY146C 1:50 /
Immunofluorescence
Enlarge image
REY146C mAb in HEK293T transfected cells
To confirm that REY146C mAb recognized mouse FRA2 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged mFRA2 expressed in Hek293T was performed. Cytospin preparation of mouse FRA1 and mouse FOSB transfected cells was used as negative controls to confirm the specificity of the antibody. Labeling with the anti-V5, anti-FOSB and anti-FRA1 antibodies confirmed the efficiency of the transfections.
Enlarge image
REY146C antibody can be used to detect mouse FRA2 protein in paraffin embedded tissue samples.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
REY146C 1:100 Supernatant / Available
Immunoprecipitation
Enlarge image
REY146C mAb is able to detect mFRA2 protein by WB.
Lane 1 Mouse keratinocytes wt (100ug)
Lane 2 Mouse keratinocytes FRA2 KO (100ug)
Lane 3 Mouse epidermis wt (100ug)
Lane 4 Mouse epidermis FRA2 KO (100ug)