mAbs Against MOUSE Protein
HES1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | HS395A |
| PROTEIN: | Transcription factor HES-1 - Hairy and Enhancer of split-1 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P35428 |
| ANTIGEN USED: | HIS-mHES1 (aa93-aa282 fragment) |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2b |
| SPECIES REACTIVITY: | Mouse and human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
References
Maraver A, Fernandez-Marcos PJ, Cash TP, Mendez-Pertuz M, Dueñas M, Maietta P, Martinelli P, Muñoz-Martin M, Martínez-Fernández M, Cañamero M, Roncador G, Martinez-Torrecuadrada JL, Grivas D, de la Pompa JL, Valencia A, Paramio JM, Real FX, Serrano M. NOTCH pathway inactivation promotes bladder cancer progression. J Clin Invest. 2015 Feb;125(2):824-30.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | HS395A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | HS395A | Neat | supernatant | / | |||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | HS395A | 1:50 | purified | / | Available | ||||
Enlarge image
- HS395A is able to detect mouse Hes1 protein in immunocytochemistry
- To confirm that HS395A mAb recognizes mouse HES1 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged mHES1 expressed in HEK293T cell line was performed. Anti-V5 antibody was used as positive control. HEK-FGFR1-v5 vere used as a negative control.
Enlarge image
- HS395A mAb can be used to detect Hes1 protein in mouse paraffin tissues
Enlarge image
- HS395A mAb can be used to detect mouse Hes1 protein by immunofluorescence