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mAbs Against MOUSE Protein

Ly9 (recognizes Ly9.1 and Ly9.2)

CONTACT INFORMATION:Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona
TYPE:Mouse anti-mouse
CLONE NAME:mLy9.10.19
PROTEIN:mouse Ly9 (CD229)
ANTIGEN USED:300.19 pre-B cell line
FUSION PARTNER:NS1 cells
ISOTYPE:IgG1 (kappa)
SPECIES REACTIVITY:Mouse
PREPARATION AND STORAGE:Aliquot and store at 4ºC. Do not freeze
APP RECOMMENDED:Flow cytometry, ELISA, IP
APP NO RECOMMENDED:IHQ-paraffin, WB
APP NO TESTED:IF

APPLICATIONS

WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Immunoprecipitation
mLy9.10.19 2 ug/ml / Unrelated mAb 110-120 120 Not done
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IP of mLy9.10.19
Immunoprecipitation with 1 ml Supernetant, using biotin labeled 300.19 cells and Sepharose beads with protein G.
OTHERS
OTHERS Title Description Protocol
test
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Thymus Ly9 KO mouse
Flow cytometry with thymocytes of a Balb/c Ly9 KO mouse using anti-Mouse PE conjugated as secondary antibody
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Thymus Ly9 Balb/c WT mouse
Flow cytometry with thymocytes of a Balb/c Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody
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Thymus Ly9 C57BL/6 WT mouse
Flow cytometry with thymocytes of a C57BL/6 Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody