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mAbs Against MOUSE Protein

mouse Ly9.1 (allotype 1 specific)

Antibody Validated
CONTACT INFORMATION:Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona
STATUS:Validated
TYPE:Mouse anti-mouse
CLONE NAME:mLy9.10.33
PROTEIN:mouse Ly9 (CD229)
ANTIGEN USED:300.19 pre-B cell line
FUSION PARTNER:NS1 cells
ISOTYPE:IgG1 (kappa)
SPECIES REACTIVITY:Mouse
PREPARATION AND STORAGE:Aliquot and store at 4ºC. Do not freeze
APP RECOMMENDED:Flow cytometry, ELISA, IP, IF
APP NO RECOMMENDED:IHQ-paraffin, WB

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Immunofluorescence
mLy9.10.33 1/50 0.34 mg/ml No / Membrane Not done
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Immunofluorescence staining
Double staining in frozen mouse spleen. In red, Biotin mLy9.10.33 (with Streptavidin A555); in greeen, mIgM FITC conjugated
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Immunoprecipitation
Recommended Result obtained is satisfactory. The reagent can be use in this application
mLy9.10.33 2 ug/ml / Unrelated mAb 110-120 120 Not done Available
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IP of mLy9.10.33
Immunoprecipitation with 1 ml Supernetant, using Sepharose beads with protein G and biotin labeled 300.19 cells
FLOW CYTOMETRY
FLOW CYTOMETRY Clone Dilution +/- control Type of fluorocrom Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
10.33 clA 1/2 Thymus Ly9 WT mice / Thymus Ly9 KO mice Phycoerythrin
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Thymus Ly9 KO Balb/c mouse
Flow cytometry with thymocytes of a Balb/c Ly9 KO mouse using anti-Mouse PE conjugated as secondary antibody.
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Thymus Ly9 WT Balb/c mouse
Flow cytometry with thymocytes of a Balb/c Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody.