mAbs Against MOUSE Protein
mouse Ly9.1 (allotype 1 specific)
| STATUS: |  |
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| CONTACT INFORMATION: | Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona |
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| STATUS: | Validated |
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| TYPE: | Mouse anti-mouse |
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| CLONE NAME: | mLy9.10.33 |
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| PROTEIN: | mouse Ly9 (CD229) |
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| ANTIGEN USED: | 300.19 pre-B cell line |
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| FUSION PARTNER: | NS1 cells |
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| ISOTYPE: | IgG1 (kappa) |
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| SPECIES REACTIVITY: | Mouse |
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| PREPARATION AND STORAGE: | Aliquot and store at 4ºC. Do not freeze |
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| APP RECOMMENDED: | Flow cytometry, ELISA, IP, IF |
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| APP NO RECOMMENDED: | IHQ-paraffin, WB |
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Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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| Immunofluorescence |
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| mLy9.10.33 |
1/50 |
0.34 mg/ml |
No |
|
/ |
Membrane |
Not done |
|
Enlarge image
- Immunofluorescence staining
- Double staining in frozen mouse spleen. In red, Biotin mLy9.10.33 (with Streptavidin A555); in greeen, mIgM FITC conjugated
WB Techniques
| WB Techniques |
Clone |
Dilution |
Antibody concentration |
+/- control |
Expected MW |
Observed Mw |
Positivity in other species |
Protocol |
| Western Blotting |
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| Immunoprecipitation |
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Recommended Result obtained is satisfactory. The reagent can be use in this application | mLy9.10.33 |
|
2 ug/ml |
/ Unrelated mAb |
110-120 |
120 |
Not done |
Available |
Enlarge image
- IP of mLy9.10.33
- Immunoprecipitation with 1 ml Supernetant, using Sepharose beads with protein G and biotin labeled 300.19 cells
FLOW CYTOMETRY
| FLOW CYTOMETRY |
Clone |
Dilution |
+/- control |
Type of fluorocrom |
Protocol |
Recommended Result obtained is satisfactory. The reagent can be use in this application | 10.33 clA |
1/2 |
Thymus Ly9 WT mice / Thymus Ly9 KO mice |
Phycoerythrin |
|
Enlarge image
- Thymus Ly9 KO Balb/c mouse
- Flow cytometry with thymocytes of a Balb/c Ly9 KO mouse using anti-Mouse PE conjugated as secondary antibody.
Enlarge image
- Thymus Ly9 WT Balb/c mouse
- Flow cytometry with thymocytes of a Balb/c Ly9 WT mouse using anti-Mouse PE conjugated as secondary antibody.
