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mAbs Against MOUSE Protein


STATUS:Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
TYPE:rat monoclonal
PROTEIN:NGFR Tumor necrosis factor receptor superfamily member 16
ANTIGEN USED:His-mNGFR (276-427aa) protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze


Low affinity neurotrophin receptor which can bind to mature NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF (proNGF), BDNF (proBDNF) and NTF3 (proNT3) with high affinity, and has much lower affinity for mature NGF and BDNF. Plays an important role in differentiation and survival of specific neuronal populations during development. Can mediate cell survival as well as cell death of neural cells.


IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
NORI138B neat supernatant /
Paraffin tissue
Enlarge image
NORI138B mAb is able to detect human and mouse NGFR protein in immunocytochemistry.
To confirm if NORI138B is specific for human and mouse NGFR protein, immunocytochemistry was performed on frozen cytospin preparations of HEK293 cells tranfected with pcmv6-mNGFR-MYC-DDK and pLenti-hNGFR-mGFP. Labeling with the anti-GFP and anti-MYC mAbs confirmed the efficiency of transfection.