mAbs Against MOUSE Protein
P15
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti mouse |
| CLONE NAME: | PAT56B |
| PROTEIN: | Mouse cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (Cdkn2b) |
| ANTIGEN USED: | HIS-GST-mp15 recombinant protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, also known as p15Ink4b protein, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases by cyclin D, thus the encoded protein functions as a cell growth regulator that inhibits cell cycle G1 progression.
References
Muñoz-Espín D, Cañamero M, Maraver A, Gómez-López G, Contreras J, Murillo-Cuesta S, Rodríguez-Baeza A, Varela-Nieto I, Ruberte J, Collado M, Serrano M. Programmed cell senescence during mammalian embryonic development. Cell. 2013 Nov 21;155(5):1104-18.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | PAT56B | 1:2 | supernatant | mouse fibrosarcoma / | nuclear | ||||
| Immunofluorescence | |||||||||
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- mp15 antibody (PAT56B) in transfected cells
- Nuclear and cytoplasmic staining of PAT56B mAb in transfected HEK293-V5-mp15 cells. No reactivity with mp16 was found. Anti-V5 was used as a positive control.
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- PAT56B antibody in mouse paraffin tissues.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | PAT56B | Neat supernatant | supernatant | / | 14kDa | 14kDa | Available | |
| Immunoprecipitation | ||||||||
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- Western Blott of PAT56B antibody in mouse and human extracts
- Lane 1 Hek-V5-p15 (30цg) (+)
Lane 2 Hek-v5-p16 (30цg) (-)
Lane 3 Hela cell line (30цg) (-)
Lane 4 NIH/3T3 cell line (100цg) (-)
Lane 5 COLO cell line (100цg) (-)
Lane 6 Mefs (100цg) (+)
Lane 7 Mefs (100цg) (+)
Lane 8 Mefs (100цg) (-)
Lane 9 Ramos cell line (100цg) (-)
Anti-Tubulin was used as loading control.