mAbs Against MOUSE Protein
SA1 - STAG1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | rat anti mouse |
| CLONE NAME: | SUSI 63B |
| PROTEIN: | Cohesin subunit SA-1 |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/Q9D3E6 |
| ANTIGEN USED: | mSA1-MBP recombinant protein (N terminal fragment 225 aa) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse and human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | undiluted | supernatant | / | nuclear | ||||
| Paraffin tissue | |||||||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI63B | Neat | supernatant | / | Available | ||||
Enlarge image
- SUSI63B is able to detect mouse SA-1 protein in immunocytochemistry
Enlarge image
- SUSI63B mAb can be used to detect SA-1 protein by immunofluorescence in mouse cells
- Mouse embryo fibroblasts (MEFs) from wild type or Stag1 KO embryos fixed in paraformaldehyde.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SUSI 63B | undiluted supernatant | supernatant | / | 144 kDa | 144kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- SUSI 63B mAb is able to detect mouse SA1 protein by WB
- Lane 1 Whole mouse embryo fibroblast Mef extract SA1 WT (10ug) (+)
Lane 2 Whole mouse embryo fibroblast Mef extract SA1 KO (10ug) (-)
Lane 3 Whole mouse embryo fibroblast Mef extract SA2 WT (10ug) (+)
Lane 4 Whole mouse embryo fibroblast Mef extract SA2 KO (10ug) (+)