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mAbs Against MOUSE Protein

SUZ12

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti mouse
CLONE NAME:124C
PROTEIN:human SUZ12
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/197333809
ANTIGEN USED:MBP-SUZ12 recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:IHQ-paraffin, IHQ-frozen, IF, WB
APP NO TESTED:Flow cytometry, IP

DESCRIPTION

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Deregulated expression of the polycomb-group protein SUZ12 target genes characterizes mantle cell lymphoma. Martín-Pérez D, Sánchez E, Maestre L, Suela J, Vargiu P, Di Lisio L, Martínez N, Alves J, Piris MA, Sánchez-Beato M. Am J Pathol 2010 Aug;177(2):930-42. http://www.ncbi.nlm.nih.gov/pubmed?term=maestre%20l%20sanchez-beato%20m

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
124C 1:50 purified 1mg/ml Discovery Xt CC1 OmniMap Ventana testicle / nuclear human
Immunofluorescence
Enlarge image
Anti-SUZ12 antibody (124C) in mouse testicle paraffin section
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
124C Undiluted supernatant NS-1 cell line / 82kDa 82kDa Available
Immunoprecipitation
Enlarge image
Western blotting of anti-SUZ12 (124C) in different mouse cell lines and tissues
GAPDH was used as loading control
Lane 1 mouse thymus (100ug)
Lane 2 BW5147.3 cell line (100ug)
Lane 3 NIH-3T3 cell line (100ug)
Lane 4 CH12 cell line (100ug)
Lane 5 300.19 cell line (100ug)
Lane 6 mouse spleen lymphoprep (100ug)
Lane 7 NS-1 cell line (100ug)
Lane 8 mouse testis (100ug)
Lane 9 mouse spleen (100ug)