mAbs Against MOUSE Protein
SUZ12
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti mouse |
| CLONE NAME: | 124C |
| PROTEIN: | human SUZ12 |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/197333809 |
| ANTIGEN USED: | MBP-SUZ12 recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-paraffin, IHQ-frozen, IF, WB |
| APP NO TESTED: | Flow cytometry, IP |
Description
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Publication describing antibody characterization/validation
Deregulated expression of the polycomb-group protein SUZ12 target genes characterizes mantle cell lymphoma. Martín-Pérez D, Sánchez E, Maestre L, Suela J, Vargiu P, Di Lisio L, Martínez N, Alves J, Piris MA, Sánchez-Beato M. Am J Pathol 2010 Aug;177(2):930-42. http://www.ncbi.nlm.nih.gov/pubmed?term=maestre%20l%20sanchez-beato%20m
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 124C | 1:50 | purified 1mg/ml | Discovery Xt CC1 OmniMap | Ventana | testicle / | nuclear | human | |
| Immunofluorescence | |||||||||
Enlarge image
- Anti-SUZ12 antibody (124C) in mouse testicle paraffin section
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 124C | Undiluted | supernatant | NS-1 cell line / | 82kDa | 82kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- Western blotting of anti-SUZ12 (124C) in different mouse cell lines and tissues
- GAPDH was used as loading control
Lane 1 mouse thymus (100ug)
Lane 2 BW5147.3 cell line (100ug)
Lane 3 NIH-3T3 cell line (100ug)
Lane 4 CH12 cell line (100ug)
Lane 5 300.19 cell line (100ug)
Lane 6 mouse spleen lymphoprep (100ug)
Lane 7 NS-1 cell line (100ug)
Lane 8 mouse testis (100ug)
Lane 9 mouse spleen (100ug)