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mAbs Against MOUSE Protein

TRF1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti mouse
CLONE NAME:572C
PROTEIN:Mus musculus Telomeric repeat binding protein 1
PROTEIN WEB:http://www.uniprot.org/uniprot/P70371
ANTIGEN USED:mTRF1-His
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
572C Undiluted supernatant Supernatant / nuclear
Paraffin tissue
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
572C Undiluted supernatant Supernatant / nuclear Available
Enlarge image
Anti-TRF1 antibody (572C) in transfected cells.
To confirm that 572C mAb recognized mouse TRF1 protein, immunohistochemistry on frozen cytospins preparations of mTRF1 expressed in Hek293T was performed. Hek293T-mTRF2-GFP was used as negative control. Anti-TRF1 Abcam antibody was used as positive control.
Enlarge image
572C mAb is able to detect mouse TRF1 protein by immunofluorescence.
mTRF1- red
GFP - green
nuclei - blue
Enlarge image
572C mAb is able to detect mouse TRF1 protein in a mouse blastocyste.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
572C Undiluted supernatant Supernatant / 47kDa 50kDa Available
Immunoprecipitation
Enlarge image
572C mAb is able to detect mouse TRF1 protein by WB
Lanes:

Lane 1 IPS-GFP-mTRF1 homo (100ug) (+)
Lane 2 IPS-GFP-mTRF1 heter (100ug) (+/-)
Lane 3 IPS-sh mTRF1 (100ug) (-)
Lane 4 IPS-sh mTRF1 puro (100ug) (-)
Lane 5 Hek-mTRF1 (20ug) (+)
Lane 6 Hek-FGFR2 (20ug) (-)
Lane 7 NIH-3T3 (100ug) (+)
Enlarge image
572C mAb is able to detect mouse TRF1 protein by WB.
Lanes:

Lane 1 Mefs TRF1 +/+ nuclear extract (30ug) (+)
Lane 2 Mefs TRF1 KO nuclear extract (30ug) (-)

Image kindly provided by Ianire Garrobo