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mAbs Against HUMAN Protein

AID

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:JUA51E
PROTEIN:Activation induced cytidine deaminase
PROTEIN WEB:http://www.hprd.org/summary?hprd_id=05585&isoform_id=05585_1&isoform_name=Isoform_1
ANTIGEN USED:EVDDLRDAFRMLGF peptide
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

Maturation of the antibody repertoire is mediated by two different mechanisms: class-switch recombination (CSR) and somatic hypermutation (SHM). These two processes are T cell dependent and occur in the germinal centres of secondary lymphoid organs. CSR leads to the production of antibodies of different isotypes whereas SHM leads to the selection of B cells expressing a BCR with high affinity for antigen. The activation-induced cytidine deaminase (AID) was recently shown to play a key role in these two mechanisms, demonstrating for the first time that these maturation processes share a common mechanism. There is evidence that AID is involved in the somatic DNA alterations required for CSR and SHM.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
JUA51E 1:2 Supernatant Tris-EDTA Novolink Tonsil / nuclear and cytoplasmic
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
JUA51E undiluted supernatant Tris-EDTA Tonsil / nuclear and cytoplasmic Available
Enlarge image
JUA51E mAb in transfected cells.
Cytoplasmic staining on frozen cytospin preparations of transfected HEK293T/AID cells using antibody JUA51E. HEK293T/p27 transfected cells were used as negative control. Anti-HA and anti-AID (EK2 clone) were used as positive controls.
Enlarge image
JUA51E mAb in paraffin sections.
Enlarge image
AID (JUA51E) immunofluorecence in human tonsil
Anti-AID (EK2) was used as positive control.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
JUA51E Undiluted Supernatant Daudi cell line / 24kDa 26kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of AID (JUA51E) using different human cell lines.
Lane 1 Hek-AID-HA (30ug) (+)
Lane 2 Hek-AID-HA (30ug) (+)
Lane 3 Hek-PSF1 (30ug) (-)
Lane 4 Ramos cell line (100ug) (-)
Lane 5 Jurkat cell line (100ug) (-)
Lane 6 Daudi cell line (100ug) (+)
Lane 7 BL2 cell line (100ug) (-)
Lane 8 Alewife cell line (100ug) (-)
Lane 9 Human tonsil (100ug) (-)