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mAbs Against HUMAN Protein

C-Myb

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti human
CLONE NAME:ANA236
PROTEIN:Homo sapiens v-myb myeloblastosis viral oncogene homologue avian, c-myb
PROTEIN WEB:http://www.hprd.org/summary?hprd_id=01810&isoform_id=01810_3&isoform_name=Isoform_1
ANTIGEN USED:HIS-c-Myb (aa402-aa640 fragment)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
ANA236 1:80 purified Bone marrow / Available
Immunofluorescence
Enlarge image
Validation of ANA236B monoclonal antibody in transfected cells
Nuclear and cytoplasmic staining on frozen cytospin preparations of transfected HEK293T-C-myb cells using antibody ANA236B. HEK-A-myb and HEK-B-myb transfected cells were used as negative control.
Enlarge image
ANA236B staining in human paraffin sections
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
ANA236 1:50 PURIFIED Jurkat / YT 72kDa 72kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of C-MYB (ANA236) using different human cell lines.
Lane 1 DAUDI (150 ug) (-)
Lane 2 RAMOS (150 ug) (-)
Lane 3 RAJI (150 ug) (-)
Lane 4 KARPAS299 (150 ug) (+)
Lane 5 HUT78 (150 ug) (-)
Lane 6 JURKATT (150 ug) (+)
Lane 7 MT2 (150 ug) (+)
Lane 8 SUP-T1 (150 ug) (+)
Lane 9 YT (150 ug) (-)
Lane 10 Molecular Weight