EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

CD8A

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti human
CLONE NAME:NOR132
PROTEIN:Human full length CD8A protein
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/omim/186910
ANTIGEN USED:Full lenght HIS-CD8
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4ºC. Do not freeze
APP RECOMMENDED:IHQ-frozen, IHQ-paraffin, IF, WB, IP
APP NO TESTED:Flow cytometry

DESCRIPTION

CD8 (cluster of differentiation 8) is a transmembrane glycoprotein that serves as a co-receptor for the T cell receptor (TCR). Like
the TCR, CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. CD8
antigen is strongly expressed on human cytotoxic T cells and thymocytes, and is also expressed on a subset of NK cells.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
NOR132 neat supernatan none goar anti rat HRP DAKO1:100 tonsil / membrane
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
NOR132 1:20 supernatan 30min ER1 Novolink kit tonsil / pancreas membrane Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
NOR132 neat supernatan Tris-EDTA Goat anti mouse IgGra Alexa tonsil / membrane Available
Enlarge image
IHC of CD8 (NOR132H) and CD8 (C8/144B) in transfected cells.
Antibody CD8 (NOR132H) was characterized by immunohistochemistry on transfected Hek-pcDNA3-V5-CD8 cells. This antibody specificity has been compared with anti-CD8 (clone C8/144B)
Enlarge image
Expression of CD8 (NOR132) and CD8 (C8/144B) in frozen tonsil
Enlarge image
Expression of CD8 (NOR132) and CD8 (C8/144B) on paraffin tonsil, spleen, testicle and pancreas
Enlarge image
Expression of CD8 (NOR132) and CD8 (C8/144B) in paraffin tonsil using immunofluorescence technique
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
NOR132 NEAT supernatan Tonsil / Jurkat 30kDa 30kDa Human Available
Inconclusive Result obtained is not completely satisfactory. More testing are needed before using these reagents in this application
C8/144B NEAT supernatan Tonsil / Jurkat 30kDa 30kDa Human Available
Immunoprecipitation
Recommended Result obtained is satisfactory. The reagent can be use in this application
NOR132 NEAT supernatan Tonsil / Jurkat 30kDa 30kDa Human Available
Inconclusive Result obtained is not completely satisfactory. More testing are needed before using these reagents in this application
C8/144B NEAT supernatan Tonsil / Jurkat 30kDa 30kDa Human Available
Enlarge image
Expression of CD8 (NOR132) and CD8 (C8/144B) by WB
Lanes
Lane 1 Hek-CD8 (30цg) (+)
Lane 2 Hek-Bcl2 (30цg) (-)
Lane 3 Tonsil (100цg) (+)
Lane 4 Human Thymus (100цg) (+)
Lane 5 Human spleen (100цg) (+)
Lane 6 Mouse spleen (100цg) (-)
Lane 7 Peripheric blood (100цg) (+)
Lane 8 JURKAT (100цg) (-)
Lane 9 TOLEDO (100цg) (-)
Enlarge image
IP of CD8 (NOR132) and CD8 (C8/144B) mAbs
A. Immunoprecipitation of protein extracts from HEK-CD8, HEK-Bcl2, Tonsil and Jurkat with commercial CD8 mAb (clone C8/144B) (used as neat supernatant) followed by western blotting with CD8 NOR132H.
B. Immunoprecipitation of protein extracts from HEK-CD8, HEK-Bcl2, Tonsil and Jurkat with CD8 NOR132H (used as neat supernatant) followed by western blotting with CD8 NOR132H
C. Immunoprecipitation of protein extracts from HEK-CD8, HEK-Bcl2, tonsil and Jurkat with commercial CD8 mAb (clone C8/144B) (used as neat supernatant) followed by western blotting with CD8 C8/144B.
D. Immunoprecipitation of protein extracts from HEK-CD8, HEK-Bcl2, Tonsil and Jurkat with CD8 NOR132H (used as neat supernatant) followed by western blotting with CD8 C8/144B.