EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

CDK6

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:98D
PROTEIN:Cyclin dependent kinase 6
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/AAD43504.1
ANTIGEN USED:His-mCDK6 recombinant protein (full length)
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
98D 1:20 neat supernatant Tris-EDTA Novolink kit / mouse Available
Immunofluorescence
Enlarge image
98D mAb in trasfected cells
To confirm that 98D mAb recognized CDK6 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged human CDK6 expressed in Hek293T was performed. Cytospin preparation of V5-tagged mouse p21 protein was used as a negative control. Labeling with the anti-hCDK6 mAb confirmed the efficiency of transfection.
Enlarge image
98D antibody can be used to detect human CDK6 protein in paraffin embedded tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
98D undiluted supernatant / 37kDa 37kDa mouse Available
Immunoprecipitation
Enlarge image
Western Blotting of CDK6 (98D) using different human cell lines.
Lane 1 Hek-CDK6 (20ug)
Lane 2 Hek-BCL7c (20ug)
Lane 3 HeLa (200ug)
Lane 4 Jurkat (200ug)
Lane 5 K562 (200ug)
Lane 6 Tonsil (200ug)