mAbs Against HUMAN Protein
CENPC1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | AL61A |
| PROTEIN: | Centromeric protein c1 |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/NP_001803.2 |
| ANTIGEN USED: | hCENPC Nterm-GST |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.[provided by RefSeq, Jul 2008].
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | AL61A | Neat supernatant | Hela / | nuclear | Available | ||||
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- AL61A mAb in transfected cells
- Nuclear and cytoplasmic staining on frozen cytospin preparations of transfected HEK293T/CENPC1 cells using antibody AL61A. Labeling with the anti-CENPC pAb confirmed the efficiency of transfection. HEK293T/ZBTB32 transfected cells were used as a negative control.
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- Immunofluorescence of anti-CENPC1 mAb AL61A.
- CENPC1 (AL61A) nuclear protein in red and DAPI staining in blue in Hela and Jurkat human cell lines.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
| Immunoprecipitation | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | AL61A | Hela nuclear extract / | Available | |||||
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- CENPC1 Immunoprecipitation by using AL61A mAb.
- Western blotting results of an immunoprecipitation of HELA nuclear extract by using clon AL61A and botting with another anti-CENPC1 clon (AL159A).
Lane 1 input 1%
Lane 2 HeLa nuclear extract (+)
Lane 3 HeLa nuclear extract (+)
Lane 4 Control (-)