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mAbs Against HUMAN Protein

CENPC1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:AL61A
PROTEIN:Centromeric protein c1
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/NP_001803.2
ANTIGEN USED:hCENPC Nterm-GST
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.[provided by RefSeq, Jul 2008].

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
AL61A Neat supernatant Hela / nuclear Available
Enlarge image
AL61A mAb in transfected cells
Nuclear and cytoplasmic staining on frozen cytospin preparations of transfected HEK293T/CENPC1 cells using antibody AL61A. Labeling with the anti-CENPC pAb confirmed the efficiency of transfection. HEK293T/ZBTB32 transfected cells were used as a negative control.
Enlarge image
Immunofluorescence of anti-CENPC1 mAb AL61A.
CENPC1 (AL61A) nuclear protein in red and DAPI staining in blue in Hela and Jurkat human cell lines.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Immunoprecipitation
Recommended Result obtained is satisfactory. The reagent can be use in this application
AL61A Hela nuclear extract / Available
Enlarge image
CENPC1 Immunoprecipitation by using AL61A mAb.
Western blotting results of an immunoprecipitation of HELA nuclear extract by using clon AL61A and botting with another anti-CENPC1 clon (AL159A).

Lane 1 input 1%
Lane 2 HeLa nuclear extract (+)
Lane 3 HeLa nuclear extract (+)
Lane 4 Control (-)