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mAbs Against HUMAN Protein

CPEB4

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti-human
CLONE NAME:ERE93C
PROTEIN:mouse CPEB4 (Cytoplasmic polyadenylation element-binding protein 4)
PROTEIN WEB:http://www.uniprot.org/uniprot/Q17RY0
ANTIGEN USED:HIS-GST-mCPEB4 (1-375aa) recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

REFERENCES

Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS.
Nat Commun. 2016 Nov 18; 7:13418.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
ERE93C neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
ERE93C 1:1500 purified / Available
Immunofluorescence
Enlarge image
ERE93CmAb in HEK293T transfected cells.
Anti-Flag was used as positive control. HEK-TOX-Flag transfected cells were used as negative control.
Enlarge image
ERE93C staining in human paraffin sections.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
ERE93C melanoma tissue / Available
Immunoprecipitation
Enlarge image
Western Blotting of CPEB4 (ERE93C) using different human cell lines.
Predicted molecular weight: 80kDa
Observed molecular weight: 80kDa

Lanes
Lane 1 Foreskin melanocytes (prep 1)
Lane 2 Foreskin melanocytes (prep 2)
Lane 3 Melanoma cell line SK-Mel-5
Lane 4 Melanoma cell line SK-Mel-28
Lane 5 Melanoma cell line SK-Mel-29
Lane 6 Melanoma cell line SK-Mel-103
Lane 7 Melanoma cell line UACC-62
Lane 8 RWP-1 cell line (pancreatic cancer)
Lane 9 HeLa cell line (cervical cancer)
Lane 10 U251 cell line (glioma)
Lane 11 Melanoma cell line SK-Mel-28 transduced with pLK.O1_shControl
Lane 12 Melanoma cell line SK-Mel-28 transduced with pLK.O1_shCPEB4
Lane 13 Melanoma cell line SK-Mel-28 transduced with pLV empty vector
Lane 14 Melanoma cell line SK-Mel-28 transduced with pLV-CPEB4

Image provided by Eva Pérez.