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mAbs Against HUMAN Protein

EED

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:163C
PROTEIN:Human full length EED (Embryonic ectoderm development)
PROTEIN WEB:http://www.hprd.org/alternate?hprd_id=09343&isoform_id=09343_1&isoform_name=Isoform_1
ANTIGEN USED:His-EED full length protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG2a
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Active Motif

DESCRIPTION

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
163C 1:600 Supernatant Tris-EDTA Novolink Tonsil / Nuclear Mouse
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
163C 1:40 Supernatant Tris-EDTA Alexa 488 Tonsil / Nuclear Available
Enlarge image
EED antibody (163C) in transfected cells.
Validation of EED 163C mAb in HEK293T-EED transfected cells. Hek-MNDA transfected cells was used as negative control. Anti-v5 was used as positive control.
Enlarge image
EED (163C) expression in human paraffin sections.
In Human lymph node, thymus, testicle and bone marrow EED protein is expressed in the nucleus.
Enlarge image
EED (163C) immunofluorecence in human tonsil.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Not recommended Reagent discarded for this application
163C undiluted supernatant / 50kDa Available
Immunoprecipitation
Enlarge image
Anti-EED (163C) antibody in Western Blott
Lane 1 Hek-EED (200ug)
Lane 2 Hek-Bcl7B (200ug)
Lane 3 Tonsil (200ug)
Lane 4 U2OS (200ug)
Lane 5 L428 (200ug)
Lane 6 Hela (200ug)
Lane 7 Molt-4 (200ug)
Lane 8 Raji (200ug)