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mAbs Against HUMAN Protein

HJURP

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:HOPE169E
PROTEIN:Holliday junction recognition protein (HJURP)
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/83816964
ANTIGEN USED:HJURP-GST recombinant full length protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

Holliday junction recognition protein (HJURP), a centromere protein-A (CENP-A) histone chaperone, mediates centromere-specific assembly of CENP-A nucleosome, contributing to high-fidelity chromosome segregation during cell division.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
HOPE169E neat supernatant Tonsil /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
HOPE169E 1:2 supernatant Tris-EDTA Novolink kit Tonsil / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
HOPE169E Tonsil / Nuclear Available
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Validation of HOPE169E monoclonal antibody in transfected cells
Nuclear and cytoplasmic staining on frozen cytospin preparations of transfected HEK293T-MRFP-HJURP cells using antibody HOPE169E. HEK-MRFP-A-myb were used as negative control. Anti-MRFP was used as positive control.
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Anti-HJURP antibody in human frozen tonsil.
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Anti-HJURP in paraffin sections.
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Anti-HJURP in tonsil paraffin section.
HOPE169E is represented in green
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
HOPE169E Neat Supernatant Daudi / 83kDa 83kDa Available
Immunoprecipitation
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HOPE169E in western blotting
Lane 1 Human Kidney (100ug) (+)
Lane 2 Human colon (100ug) (-)
Lane 3 K562 cell line (100ug) (+)
Lane 4 Daudi cell line (100ug) (+)
Lane 5 Raji cell line (100ug) (+)