EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

LMO2

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:1A9-1
PROTEIN:human full length LMO2
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/214832074
ANTIGEN USED:GST-LMO2
FUSION PARTNER:K6H6B5
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze.
APP RECOMMENDED:IHQ-paraffin, WB, IF
APP NO TESTED:IHQ-frozen, IP, Flow cytometry

DESCRIPTION

LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms. LMO2 protein is expressed as a nuclear marker in normal germinal-center (GC) B cells and GC-derived B-cell lines and in a subset of GC-derived B-cell lymphomas. LMO2 is expressed in erythroid and myeloid precursors and in megakaryocytes and also in lymphoblastic and acute myeloid leukemias. It is rarely expressed in mature T, natural killer (NK), and plasma cell neoplasms and is absent from nonhematolymphoid tissues except for endothelial cells.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Yasodha Natkunam, Shuchun Zhao, David Y. Mason, Jun Chen, Behnaz Taidi, Margaret Jones, Anne S. Hammer, Stephen Hamilton Dutoit, Izidore S. Lossos and Ronald Levy. The oncoprotein LMO2 is expressed in normal germinal-center B cells and in human B-cell lymphomas. 2007 109: 1636-1642.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
1A9-1 1:4 Tris-EDTA Leica Bond Max Tonsil /
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
1A9-1 1:100 supernatant Tonsil / Available
Enlarge image
LMO2 antibody (1A9-1) in transfected cells
Cytoplasmic staining of LMO2 mAb in transfected HEK-V5-LMO2 cells. Anti-V5 mAb was used as positive control.
Enlarge image
Anti-LMO2 in tonsil paraffin sections
Enlarge image
LMO2 (1A9-1) immunofluorescence in human paraffin tonsil.
Anti-LMO2 (1A9-1) is represented in red. DAPI in blue.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
1A9-1 Neat Supernatant Ramos cell line / Hela cell line 18kDa 18kDa Available
Immunoprecipitation
Enlarge image
Anti-LMO2 in western blotting technique
Tubulin was used as loading control.

Lane 1 Transfected Hek-pcdna3-V5-LMO2 (20ug) (+)
Lane 2 Transfected Hek-pcdna3-V5-Bcl10 (20ug) (-)
Lane 3 SUDHL6 cell line (200ug) (+)
Lane 4 Ocil19 cell line (200ug) (+)
Lane 5 Ocily3 cell line (200ug) (-)
Lane 6 Raji cell line (200ug) (-)
Lane 7 Ramos cell line (200ug) (+)
Lane 8 HeLa cell line (200ug) (-)