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mAbs Against HUMAN Protein

MNDA

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:253A
PROTEIN:Human full length MNDA
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/omim/159553
ANTIGEN USED:MNDA-GST-HIS recombinant protein
FUSION PARTNER:splenocytes & myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4ºC. Do not freeze.
APP RECOMMENDED:IHQ-paraffin, IF, WB
APP NO RECOMMENDED:IHQ-frozen
APP NO TESTED:Flow cytometry, IP

DESCRIPTION

MNDA (myeloid cell nuclear differentiation antigen) is a 55kDa nuclear protein constitutively expressed in myelomonocytic leukemia cells, myelomonocytic cell lines, and normal peripheral blood granulocytes and monocytes. A low level of expression has also been found in a population of mantle B lymphocytes but no expression exists in germinal centre cells or plasma cells.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Kanellis G, Roncador G, Arribas A, Mollejo M, Montes-Moreno S, Maestre L, Campos-Martin Y, Ríos Gonzalez JL, Martinez-Torrecuadrada JL, Sanchez-Verde L, Pajares R, Cigudosa JC, Martin MC, Piris MA (2009). Identification of MNDA as a new marker for nodal marginal zone lymphoma. Leukemia 23(10):1847-57.http://www.ncbi.nlm.nih.gov/pubmed/19474799

REFERENCES

Kanellis G, Roncador G, Arribas A, Mollejo M, Montes-Moreno S, Maestre L, Campos-Martin Y, Ríos Gonzalez JL, Martinez-Torrecuadrada JL, Sanchez-Verde L, Pajares R, Cigudosa JC, Martin MC, Piris MA (2009). Identification of MNDA as a new marker for nodal marginal zone lymphoma. Leukemia 23(10):1847-57.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
253A neat supernatant / goat anti mouse HRP DAKO tonsil / nuclear
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
253A neat supernatant ER2 (Tris-EDTA) 15 min Novolink tonsl / nuclear Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
253A neat supernatant tonsil / nuclear Available
Enlarge image
MNDA antibody (253A) in transfected cells.
Validation of MNDA (253A) monoclonal antibody in HEK-V5-MNDA transfected cells. HEK-CMYB transfected cells was used as negative control. Anti-V5 monoclonal antibody was used as positive control.
Enlarge image
MNDA (253A) immunohistochemistry in human paraffin sections.
MNDA protein is detected in the nucleus of mature granulocyte and
monocyte cells. MNDA ia also expressed, although with less intensity, by cells occupying the marginal zone surrounding the germinal centers (GCs) in normal tonsil. The GCs of all follicles are negative for MNDA. Reactive monocytoid B cells and plasma cells do not express
MNDA
Enlarge image
MNDA (253A) immunofluorescence in human paraffin tonsil.
MNDA (253A) nuclear protein in green, CD20 membrene staining in red and DAPI in blue.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
253A neat supernatant Mino cell line / YT cell line 55kDa 48kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of MNDA (253A) using different lymphoma cell lines and transfected cells.
Lane 1 Hek-MNDA (10ug) (+)
Lane 2 Hek-CD1D (10ug) (-)
Lane 3 HL60 (100ug) (-)
Lane 4 K562 (100ug) (-)
Lane 5 Z138 (100ug) (+)
Lane 6 Mino (100ug) (+)
Lane 7 YT (100ug) (-)
Lane 8 MT-2 (100ug) (+)