EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

PSGL1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:FLEG
PROTEIN:Selectin P
ANTIGEN USED:Karpas 299 cell line (Human T cell lymphoma)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:eBioscience

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
FLEG Neat supernatant Tonsil /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
FLEG Neat supernatant Tris-EDTA Tonsil / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
FLEG Neat supernatant / Available
Enlarge image
FLEG mAb in different human paraffin tissues.
Enlarge image
Immunofluorescence on human lymphocyte cells.
Anti-PSGL1 mAb (Green) and anti-CD43 mAb (red).
Enlarge image
FLEG mAb in human frozen tonsil
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
FLEG Neat supernatant SUDHL1 / KMH2 90kDa 90kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of PSGL1 (FLEG) using different cell extracts.
Lane 1 L428 cell line (100ug) (-)
Lane 2 KMH2 cell line (100ug) (-)
Lane 3 DEV cell line (100ug) (+)
Lane 4 SUDHL1 cell line (100ug) (+)
Lane 5 KARPAS cell line (100ug) (+)
Lane 6 Thymus cell line (100ug) (+)
Lane 7 human fetal liver (100ug) (-)
Lane 8 human kidney (100ug) (-)
Lane 9 HDMYZ cell line (100ug) (-)
FLOW CYTOMETRY
FLOW CYTOMETRY Clone Dilution +/- control Type of fluorocrom Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
CON6D Neat supernatant Human tonsil cells /
Enlarge image
FLEG antibody can be used to detect human PSGL1 protein by flow cytometry.
Sample: Human tonsil cell extract
Dilution: FLEG antibody was used as 50ul supernatant/one million cells/tube. Anti-CD3 antibody was diluted 1:200.