mAbs Against HUMAN Protein
PSGL1
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | FLEG |
| PROTEIN: | Selectin P |
| ANTIGEN USED: | Karpas 299 cell line (Human T cell lymphoma) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | eBioscience |
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLEG | Neat supernatant | Tonsil / | ||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLEG | Neat supernatant | Tris-EDTA | Tonsil / | Available | ||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLEG | Neat supernatant | / | Available | |||||
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- FLEG mAb in different human paraffin tissues.
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- Immunofluorescence on human lymphocyte cells.
- Anti-PSGL1 mAb (Green) and anti-CD43 mAb (red).
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- FLEG mAb in human frozen tonsil
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLEG | Neat supernatant | SUDHL1 / KMH2 | 90kDa | 90kDa | Available | ||
| Immunoprecipitation | ||||||||
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- Western Blotting of PSGL1 (FLEG) using different cell extracts.
- Lane 1 L428 cell line (100ug) (-)
Lane 2 KMH2 cell line (100ug) (-)
Lane 3 DEV cell line (100ug) (+)
Lane 4 SUDHL1 cell line (100ug) (+)
Lane 5 KARPAS cell line (100ug) (+)
Lane 6 Thymus cell line (100ug) (+)
Lane 7 human fetal liver (100ug) (-)
Lane 8 human kidney (100ug) (-)
Lane 9 HDMYZ cell line (100ug) (-)
| FLOW CYTOMETRY | Clone | Dilution | +/- control | Type of fluorocrom | Protocol |
|---|---|---|---|---|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | CON6D | Neat supernatant | Human tonsil cells / |
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- FLEG antibody can be used to detect human PSGL1 protein by flow cytometry.
- Sample: Human tonsil cell extract
Dilution: FLEG antibody was used as 50ul supernatant/one million cells/tube. Anti-CD3 antibody was diluted 1:200.