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mAbs Against HUMAN Protein

TdT

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:41A
PROTEIN:DNA nucleotidylexotransferase
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/26007035
ANTIGEN USED:HIS-TDT recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Abcam and Biolegend

DESCRIPTION

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

REFERENCES

Susan McClory, Tiffany Hughes, Aharon G Freud, Edward L Briercheck, Chelsea Martin, Anthony J Trimboli, Jianhua Yu, Xiaoli Zhang, Gustavo Leone, Gerard Nuovo, Michael A Caligiuri. Evidence for a stepwise program of extrathymic T cell development within the human tonsil. The Journal of Clinical Investigation. 2 April 2012.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
41C Neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
41C 1:10 Supernatant Thymus / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
41C 1:50 Supernatant / Available
Enlarge image
41C mAb in HEK293T-TdT transfected cells.
To confirm that 41C mAb recognized human TdT protein, immunohistochemistry on frozen cytospins preparations of human TdT expressed in HEK293T was performed. Cytospin preparation of human BTLA protein was used as a negative control.
Enlarge image
41C mAb in human thymus tissue.
Enlarge image
41C mAb can be used to detect human TDT protein by IF.
TdT (red) and DAPI (blue) in human thymus.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
41C Neat supernatant Thymus / 58kDa 58kDa Available
Immunoprecipitation
Enlarge image
41C mAb is able to detect human TdT protein by WB
Lane 1 Hek-TdT (30цg) (+)
Lane 2 Hek-Timp2 (30цg) (-)
Lane 3 Human thymus (100цg) (+)
Lane 4 Human testis (100цg) (-)