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mAbs Against HUMAN Protein

TOX

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:Rat anti human
CLONE NAME:NAN448B
PROTEIN:Thymocyte selection-associated high mobility group box protein (TOX)
PROTEIN WEB:https://www.uniprot.org/uniprot/O94900
ANTIGEN USED:His-TOX (1-250aa) recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 Neat Supernatant / nuclear
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 1:60 Supernatant Leica Tonsil / nuclear Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 1:50 Supernatant Tris-EDTA Tonsil / nuclear Available
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TOX espression in paraffin tonsil section
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TOX/KI67 double immunofluorecence in parraffin tonsil
TOX in red
Ki67 in green
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TOX/CD8 double immunohistochemistry in Mycosis Fungoides and TOX/PD1 in NLPHL
TOX brown
CD8 red
PD1 red

WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 Neat supernatant / 57kDa 57kDa Available
Immunoprecipitation
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NAN448 in WT and KO MOLT4 cell line