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mAbs Against HUMAN Protein

VPREB3

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:142B
PROTEIN:PreB lymphocyte protein 3
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/NP_037510.1
ANTIGEN USED:His-VPREB3 full length recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

The protein encoded by this gene is the human homolog of the mouse VpreB3 (8HS20) protein, and is specifically expressed in cell lines representative of all stages of B-cell differentiation. It is also related to VPREB1 and other members of the immunoglobulin supergene family. This protein associates with membrane mu heavy chains early in the course of pre-B cell receptor biosynthesis. The precise function of the protein is not known, but it may contribute to mu chain transport in pre-B cells. [provided by RefSeq, Sep 2008].

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
142B Neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
142B 1:2 Supernatant Citrate Bond Max Leica Tonsil / Available
Immunofluorescence
Enlarge image
142B mAb in cytospins preparations of HEK293T transfected cells.
To confirm that 142B mAb recognized human VPREB3 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged VPREB3 expressed in Hek293T was performed. Cytospin preparation of SUZ12 transfected cells was used as negative control. Anti-v5 antibody was used as positive control.
Enlarge image
142B mAb in paraffin embedded tissue samples.
142B antibody can be used to detect human VPREB3 protein in paraffin embedded tissue samples.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Not recommended Reagent discarded for this application
142B / Available
Immunoprecipitation