mAbs Against HUMAN Protein
VPREB3
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | 142B |
| PROTEIN: | PreB lymphocyte protein 3 |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/NP_037510.1 |
| ANTIGEN USED: | His-VPREB3 full length recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
The protein encoded by this gene is the human homolog of the mouse VpreB3 (8HS20) protein, and is specifically expressed in cell lines representative of all stages of B-cell differentiation. It is also related to VPREB1 and other members of the immunoglobulin supergene family. This protein associates with membrane mu heavy chains early in the course of pre-B cell receptor biosynthesis. The precise function of the protein is not known, but it may contribute to mu chain transport in pre-B cells. [provided by RefSeq, Sep 2008].
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 142B | Neat supernatant | / | ||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 142B | 1:2 | Supernatant | Citrate | Bond Max Leica | Tonsil / | Available | ||
| Immunofluorescence | |||||||||
Enlarge image
- 142B mAb in cytospins preparations of HEK293T transfected cells.
- To confirm that 142B mAb recognized human VPREB3 protein, immunohistochemistry on frozen cytospins preparations of V5-tagged VPREB3 expressed in Hek293T was performed. Cytospin preparation of SUZ12 transfected cells was used as negative control. Anti-v5 antibody was used as positive control.
Enlarge image
- 142B mAb in paraffin embedded tissue samples.
- 142B antibody can be used to detect human VPREB3 protein in paraffin embedded tissue samples.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Not recommended Reagent discarded for this application | 142B | / | Available | |||||
| Immunoprecipitation | ||||||||