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mAbs Against HUMAN Protein

XBP1s

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:143F
PROTEIN:human XBP1s
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/118640873
ANTIGEN USED:XBP1s C-terminus (amino-acids 165-367)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:IHQ-paraffin, IF, WB
APP NO RECOMMENDED:IHQ-frozen
APP NO TESTED:IP, Flow cytometry

DESCRIPTION

Xbox binding protein-1 (XBP-1) is a transcription factor essential for plasma cell differentiation. XBP-1 is subject to alternative RNA processing, generating two mRNA transcripts encoding the same N-terminal DNA binding domain, but different C-terminal transactivation domains. The shorter spliced transcript, designated XBP-1s, possesses enhanced transactivation potential and stability relative to the product of the unspliced transcript, designated XBP-1u. Recent studies have uncovered several functions for XBP-1 and have implicated XBP-1 overexpression in human carcinogenesis and tumour growth under hypoxic conditions. Specifically, elevated XBP-1 mRNA levels have been detected in hepatocellular carcinomas and in primary ERα-positive breast tumors.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Maestre L, Tooze R, Cañamero M, Montes-Moreno S, Ramos R, Doody G, Boll M, Barrans S, Baena S, Piris MA, Roncador G.
XBP1(S) provides a specific marker of stress response and plasma cell differentiation. Haematologica. 2009 Mar;94(3):419-22. Epub
2009 Jan 27. http://www.ncbi.nlm.nih.gov/pubmed/19176362

REFERENCES

Maestre L, Tooze R, Cañamero M, Montes-Moreno S, Ramos R, Doody G, Boll M, Barrans S, Baena S, Piris MA, Roncador G.
XBP1(S) provides a specific marker of stress response and plasma cell differentiation. Haematologica. 2009 Mar;94(3):419-22. Epub
2009 Jan 27.

Montes-Moreno S, Gonzalez-Medina AR, Rodriguez-Pinilla SM, Maestre L, Sanchez-Verde L, Roncador G, Mollejo M, García JF,
Menarguez J, Montalbán C, Ruiz-Marcellan MC, Conde E, Piris MA. Haematologica. Aggressive large B-cell lymphoma with plasma
cell differentiation: immunohistochemical characterization of plasmablastic lymphoma and diffuse large B-cell lymphoma with partial
plasmablastic phenotype. 2010 Aug; 95(8):1342-9.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
143F 1:100 supernatant 20 minutes ER2 (Tris-EDTA) Novolink kit Tonsil / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
143F supernatant / Available
Enlarge image
XBP1s antibody (143F) in transfected cells
Validation of 143F monoclonal antibody in Hek-GFP-XBP1s transfected cells. Another HEK transfected cells were used as negative control.
Enlarge image
XBP1s (143F) immunohistochemistry on human paraffin sections.
In tonsil, XBP1s protein is strongly expressed by plasma cells present in germinal center B cells and in subepithelial areas. XBP1s is also expressed in plasma cells of diferent normal tissues as bone marrow. XBP1s immunostaining of lymphoid neoplasm are represented in the images below.
Enlarge image
Expression of XBP1s (143F) antigen by lymphoid cells.
Double immunofluorescent staining shows that many of the XBP1s positive cells (green) in the germinal center cells do not co-express CD20 antigen (red).
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
143F 1:10 supernatant SK-MM-2 cell line / Ocily3 cell line 54kDa Available
Immunoprecipitation
Enlarge image
Western blotting of XBP1s (clone143F) antibody.
Specificity of the XBP1s antibody is demonstrated by detection of a single 54 kDa band in transfectants.
Lane 1 Hek-XBP1s trasfected cells (20ug) (+)
Lane 2 Human testicle (100ug) (-)
Lane 3 GST-XBP1s recombinant protein (0.1ug) (+)
Lane 4 Karpas 620 cell line (100ug) (+)
OTHERS
OTHERS Title Description Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
Immunoenzimatic staining
Enlarge image
Double XBP1s/CD138 immunoenzimatic staining
Double immunoenzymatic staining shows the expression of XBP1s (brown) and CD138 (red). This reveals that the mayority of XBP1s positive cells co-express CD138.