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mAbs Against MOUSE Protein

BCL6

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti mouse
CLONE NAME:GI191E
PROTEIN:human full length BCL6
PROTEIN WEB:http://www.hprd.org/summary?hprd_id=00180&isoform_id=00180_3&isoform_name=Isoform_1
ANTIGEN USED:pcDNA3-BCL6
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:WB, IHC-frozen, IHC-paraffin
APP NO TESTED:IP, IF, Flow Cytometry

DESCRIPTION

BCL-6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. Antibodies to this protein stain the germinal centre cells in lymphoid follicles, the follicular cells and interfollicular cells in Follicular Lymphoma, Diffuse Large B-Cell Lymphomas, and Burkitt’s lymphoma, and the majority of the Reed-Sternberg cells in Nodular Lymphocyte Predominant Hodgkin’s Disease. In contrast, anti-BCL-6 rarely stains Mantle Cell Lymphoma and MALT Lymphoma. BCL-6 expression is seen in approximately 45% of CD30+ Anaplastic Large Cell Lymphoma but is consistently absent in other peripheral T-cell lymphomas.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

García JF, García JF, Maestre L, Lucas E, Sánchez-Verde L, Romero-Chala S, Piris MA, Roncador G. Genetic Immunization: a New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J Histochem Cytochem. 2006 54(1):31-8. http://www.ncbi.nlm.nih.gov/pubmed/16046671

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
GI191E neat supernatant Discovery Xt CC1 OmniMap Ventana Lymph node / nuclear human
Immunofluorescence
Enlarge image
MAb IG191E in mouse tissue
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
GI191E supernatant 1:20 WR19L cell line / Ba/F3 cell line 78kDa 80kDa human Available
Immunoprecipitation
Enlarge image
Western Blotting of BCL6 (GI191E)
Western Blotting of BCL6 (GI191E) using different mouse and rat cell lines.
Lane 1 Ba/F3 mouse cell line (10ug) (-)
Lane 2 WR19L mouse cell line (50ug) (+)
Lane 3 PC12 rat cell line (50ug) (+)