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mAbs Against MOUSE Protein

CDK6

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti mouse
CLONE NAME:98D
PROTEIN:Cyclin dependent kinase 6
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/AAD43504.1
ANTIGEN USED:His-mCDK6 recombinant protein (full length)
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
98D neat supernatant Discovery Xt Std RiboCC Ventana lymph node / mouse
Immunofluorescence
Enlarge image
mAb 98D in mouse tissue
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
98D supernatant 3T3 cell line / 37kDa 37kDa mouse Available
Immunoprecipitation
Enlarge image
Western Blotting of CDK6 (98D) using different mouse cell and tissues extracts
Lane 1 RG15WT (200ug)
Lane 2 RG17KO (200ug)
Lane 3 3T3 cell line (200ug)