mAbs Against MOUSE Protein
CPEB4
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti-mouse |
| CLONE NAME: | ERE93C |
| PROTEIN: | CPEB4 (Cytoplasmic polyadenylation element-binding protein 4) |
| PROTEIN WEB: | http://www.uniprot.org/uniprot/Q7TN98 |
| ANTIGEN USED: | HIS-GST-mCPEB4 (1-375aa) recombinant protein |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Publication describing antibody characterization/validation
Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS.
Nat Commun. 2016 Nov 18; 7:13418.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | Neat | Supernatant | / | ||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 1:1500 | Purified | / | Available | |||||
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | Neat | Supernatant | / | Available | |||||
Enlarge image
- ERE93C mAb in transfected cells
- To confirm that ERE93C recognizes mCPEB4 immunohistochemistry on frozen cytospins preparations of GFP or Flag-tagged mCPEB1, mCPEB2, mCPEB3 and mCPEB4 expressed in Hek293T was performed. Anti-Flag and anti-GFP antibodies were used as positive controls.
Enlarge image
- ERE93C staining in mouse paraffin sections.
- ERE93C antibody can be used to detect mCPEB4 protein in mouse paraffin embedded tissue samples.
Tissue sample: mouse brain and mouse embryonic tissues.
Dilution: 1:1500
Antigen retrieval: CC1 D5+OR
Antibody incubation: 30 minutes
Detection kit used: Ventana
Enlarge image
- ERE93C antibody can be used to detect mCPEB4 protein by Immunofluorescence.
- Tissue sample: Mefs.