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mAbs Against MOUSE Protein

CPEB4

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti-mouse
CLONE NAME:ERE93C
PROTEIN:CPEB4 (Cytoplasmic polyadenylation element-binding protein 4)
PROTEIN WEB:http://www.uniprot.org/uniprot/Q7TN98
ANTIGEN USED:HIS-GST-mCPEB4 (1-375aa) recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS.
Nat Commun. 2016 Nov 18; 7:13418.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
Neat Supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
1:1500 Purified / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
Neat Supernatant / Available
Enlarge image
ERE93C mAb in transfected cells
To confirm that ERE93C recognizes mCPEB4 immunohistochemistry on frozen cytospins preparations of GFP or Flag-tagged mCPEB1, mCPEB2, mCPEB3 and mCPEB4 expressed in Hek293T was performed. Anti-Flag and anti-GFP antibodies were used as positive controls.
Enlarge image
ERE93C staining in mouse paraffin sections.
ERE93C antibody can be used to detect mCPEB4 protein in mouse paraffin embedded tissue samples.
Tissue sample: mouse brain and mouse embryonic tissues.
Dilution: 1:1500
Antigen retrieval: CC1 D5+OR
Antibody incubation: 30 minutes
Detection kit used: Ventana
Enlarge image
ERE93C antibody can be used to detect mCPEB4 protein by Immunofluorescence.
Tissue sample: Mefs.