mAbs Against MOUSE Protein
EED
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| TYPE: | mouse anti mouse |
| CLONE NAME: | 163C |
| PROTEIN: | Human full length EED (Embryonic ectoderm development) |
| PROTEIN WEB: | http://www.hprd.org/alternate?hprd_id=09343&isoform_id=09343_1&isoform_name=Isoform_1 |
| ANTIGEN USED: | His-EED full length protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cell |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Active Motif |
Description
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 163C | 1:75 | Supernatant | Discovery Xt CC1 OmniMap | Ventana | lymph node / | Nuclear | human | |
| Immunofluorescence | |||||||||
Enlarge image
- Anti-EED antibody (163C) in mouse lymp node paraffin sectionIn lymph node,
- EED protein is strongly expressed by germinal center cells.