mAbs Against MOUSE Protein
MASTL
| STATUS: |  |
|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
|---|
| STATUS: | Validated |
|---|
| TYPE: | mouse anti mouse |
|---|
| CLONE NAME: | RIPLY74C |
|---|
| PROTEIN: | Microtubule associated serine/threonine kinase-like |
|---|
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/AAH09107.1 |
|---|
| ANTIGEN USED: | GST-hMASTL recombinant full length protein |
|---|
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
|---|
| ISOTYPE: | IgG1 |
|---|
| SPECIES REACTIVITY: | Human and mouse |
|---|
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
|---|
Description
MASTL (microtubule associated serine/threonine kinase-like), also known as GW, THC2 or serine/threonine-protein kinase greatwall, is an 879 amino acid serine/threonine kinase regulates mitosis entry and maintenance. Depletion of MASTL delays the G2-to-M phase transition, is linked to a prolonged mitosis and sensitizes cells to mitotic death.
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
|---|
| Paraffin tissue |
|---|
| |
|
|
|
|
/ |
|
mouse |
|
| Immunofluorescence |
|---|
Enlarge image
- RIPLY74C mAb in transfected cells
- HEK293T/v5-hMASTL and HEK293T/V5-mMASTL cells using antibody RIPLY74C. Anti-V5 antibody was used as positive control.
