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mAbs Against MOUSE Protein

MCMV IE1

CONTACT INFORMATION:Center for Proteomics, University of Rijeka
TYPE:monoclonal
CLONE NAME:IE1.01
PROTEIN:MCMV immediate early protein 1
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/AAA74504.1
ANTIGEN USED:lysates of MCMV infected cells
FUSION PARTNER:SP2O
ISOTYPE:kappa IgG2a
SPECIES REACTIVITY:MCMV
PREPARATION AND STORAGE:affinity purified, dilluted in PBS, -20C long term, +4 short term, no azide added

DESCRIPTION

Specificity of the antibody was confirmed by mass spectrometry analysis of immunoprecipitated protein using this mAb.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
IE1.01 1:1000 MCMV infected tissue / uninfected tissue nuclear
Immunofluorescence
IE1.01 FITC MCMV infected cells / uninfected cells nuclear
Enlarge image
IHC on paraffin embedded tissues IE1.01
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FLuorescence IE1.01
Murine embryonal fibroblasts (MEFs) from Balb/c mice were infected with Smith strain of MCMV.