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mAbs Against MOUSE Protein

MCMV m06 (gp48)

CONTACT INFORMATION:Center for Proteomics, University of Rijeka
TYPE:mouse monoclonal antibody
CLONE NAME:CROMA229
PROTEIN:CCE57335.1
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/CCE57335.1
ANTIGEN USED: MHC class I immunoprecipitates from MCMV infected cells
FUSION PARTNER:SP2O
ISOTYPE:kappa IgG1
SPECIES REACTIVITY:MCMV
PREPARATION AND STORAGE:-20 C long term, short term +4 C

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.
Reusch U1, Muranyi W, Lucin P, Burgert HG, Hengel H, Koszinowski UH.
EMBO J, 1999.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171199/pdf/001081.pdf

REFERENCES

A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.
Reusch U1, Muranyi W, Lucin P, Burgert HG, Hengel H, Koszinowski UH.
EMBO J, 1999.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171199/pdf/001081.pdf

APPLICATIONS

WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
CROMA229 1:500 2 ug/mL MCMV infected cells / mock infected cells 48 48 NT
Immunoprecipitation
Enlarge image
WB m06
MCMV infected and mock infected Balb/c MEFs were lysed using RIPA buffer, separated on SDS-PAGE, transferred to PVDF membrane and immunoblotted with m06 antibody.
FLOW CYTOMETRY
FLOW CYTOMETRY Clone Dilution +/- control Type of fluorocrom Protocol
CROMA229 1:500 MCMV infected cells / mock infected cells secondary anti-mouse FITC