mAbs Against MOUSE Protein
MCMV m06 (gp48)
| STATUS: | |
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| CONTACT INFORMATION: | Center for Proteomics, University of Rijeka |
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| TYPE: | mouse monoclonal antibody |
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| CLONE NAME: | CROMA229 |
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| PROTEIN: | CCE57335.1 |
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| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/CCE57335.1 |
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| ANTIGEN USED: | MHC class I immunoprecipitates from MCMV infected cells |
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| FUSION PARTNER: | SP2O |
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| ISOTYPE: | kappa IgG1 |
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| SPECIES REACTIVITY: | MCMV |
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| PREPARATION AND STORAGE: | -20 C long term, short term +4 C |
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Publication describing antibody characterization/validation
A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.
Reusch U1, Muranyi W, Lucin P, Burgert HG, Hengel H, Koszinowski UH.
EMBO J, 1999.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171199/pdf/001081.pdf
References
A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.
Reusch U1, Muranyi W, Lucin P, Burgert HG, Hengel H, Koszinowski UH.
EMBO J, 1999.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1171199/pdf/001081.pdf
Applications
WB Techniques
| WB Techniques |
Clone |
Dilution |
Antibody concentration |
+/- control |
Expected MW |
Observed Mw |
Positivity in other species |
Protocol |
| Western Blotting |
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| CROMA229 |
1:500 |
2 ug/mL |
MCMV infected cells / mock infected cells |
48 |
48 |
NT |
|
| Immunoprecipitation |
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Enlarge image
- WB m06
- MCMV infected and mock infected Balb/c MEFs were lysed using RIPA buffer, separated on SDS-PAGE, transferred to PVDF membrane and immunoblotted with m06 antibody.
FLOW CYTOMETRY
| FLOW CYTOMETRY |
Clone |
Dilution |
+/- control |
Type of fluorocrom |
Protocol |
| CROMA229 |
1:500 |
MCMV infected cells / mock infected cells |
secondary anti-mouse FITC |
|
