EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

BCL6

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:ST42B
PROTEIN:human full length Bcl-6
PROTEIN WEB:http://www.hprd.org/summary?hprd_id=00180&isoform_id=00180_3&isoform_name=Isoform_1
ANTIGEN USED:GST-BCL6 recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:IHQ-frozen, IHQ-paraffin, WB, IF
APP NO TESTED:Flow Cytometry, IP

DESCRIPTION

BCL-6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein. Antibodies to this protein stain the germinal centre cells in lymphoid follicles, the follicular cells and interfollicular cells in Follicular Lymphoma, Diffuse Large B-Cell Lymphomas, and Burkitt’s lymphoma, and the majority of the Reed-Sternberg cells in Nodular Lymphocyte Predominant Hodgkin’s Disease. In contrast, anti-BCL-6 rarely stains Mantle Cell Lymphoma and MALT Lymphoma. BCL-6 expression is seen in approximately 45% of CD30+ Anaplastic Large Cell Lymphoma but is consistently absent in other peripheral T-cell lymphomas.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

García JF, García JF, Maestre L, Lucas E, Sánchez-Verde L, Romero-Chala S, Piris MA, Roncador G. Genetic Immunization: a New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J Histochem Cytochem. 2006 54(1):31-8. http://www.ncbi.nlm.nih.gov/pubmed/16046671

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
ST42B 1:4 supernatant None goat anti mouse DAKO Tonsil / nuclear mouse
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
ST42B 1:4 supernatant 20 min R2 (Tris-EDTA) Novolink kit Tonsil / nuclear mouse Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
ST42B neat supernatant Tris-EDTA goat anti mouse ALEXA Tonsil / nuclear mouse Available
Enlarge image
BCL6 antibody (ST42B) in HEK transfected cells.
Validation of ST42B monoclonal antibody in transfected cells. Nuclear and cytoplasmic staining on frozen cytospin preparations of transfected HEK293T-BCL6 cells using antibody ST42B. Hek-v5-CSF1R transfected cells were used as negative control.
Enlarge image
42B mAb staining in human frozen tonsil.
In tonsil, BCL6 protein is strongly expressed by germinal center B cells
Enlarge image
ST42B staining in human paraffin sections.
In tonsil, BCL6 protein is strongly expressed by germinal center B cells. Testicle section was used as a negative control.
Enlarge image
Triple immunofluorescence staining.
BCL6 (ST42B) nuclear protein in blue, PD-1 membrane staining in red and CD 57 membrane staining in green.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
ST42B neat supernatant Tonsil / 79kDa mouse Available
Immunoprecipitation
Not tested Not tested
/ mouse
Enlarge image
Western Blotting of BCL6 (ST42B) using different cell extracts.
WB analysis of cell line samples and human tonsil extract for Bcl-6.
Anti-tubulin was used as loading control.
Lane 1 Ramos cell line (100ug) (+)
Lane 2 Colo205 cell line (100ug) (-)
Lane 3 Akata cell line (100ug) (+)
Lane 4 Karpas 299 cell line (100ug) (+)
Lane 5 Toledo cell line (100ug) (+)
Lane 6 Tonsil (100ug) (+)
Lane 7 Hut78 (100ug) (-)