mAbs Against HUMAN Protein
BTLA
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse anti human |
| CLONE NAME: | FLO67B |
| PROTEIN: | human full length BTLA protein |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/145580621 |
| ANTIGEN USED: | HIS-BTLA |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| APP RECOMMENDED: | IHQ-frozen, IHQ-paraffin, IF |
| APP NO RECOMMENDED: | WB |
| APP NO TESTED: | IP, Flow cytometry |
Description
B and T lymphocyte attenuator (BTLA) has recently been identified as a new inhibitory receptor of the CD28 superfamily, with similarities to cytotoxic T lymphocyte activation antigen (CTLA)-4 and programmed death (PD)-1. Engagement of BTLA on T lymphocytes can profoundly reduce the T cell receptor (TCR)-mediated activation.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLO67B | 1:5 | supernatant | Tonsil / | Cytoplasmic | ||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLO67B | 1:50 | supernatant | 20min ER2 (Tris-EDTA) | Novolink kit | Tonsil / Kidney | Cytoplasmic | Available | |
| Immunofluorescence | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FLO67B | neat | supernatant | Tonsil / | Citoplasmic | Available | |||
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- BTLA antibody (FLO67B) in transfected cells
- Cytoplasmic staining of BTLA mAb in transfected HEK-V5-BTLA cells. Anti-V5 mAb was used as positive control. HEK-V5-B-MYB transfected cells were used as a negative control.
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- BTLA expression on human frozen tonsil.
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- FLO67B staining in human paraffin tissues.
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- BTLA (FLO67B) immunofluorescence in human paraffin tonsil.
- Anti-BTLA (FLO67B) is represented in green. anti-PD1 is represented in red. DAPI in blue.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Not recommended Reagent discarded for this application | FLO67B | neat | supernatant | / | Available | |||
| Immunoprecipitation | ||||||||
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- Anti-BTLA (FLO67B) antibody in Western Blotting
- FLO67B antibody is not working well by WB. The MW observed is around 35kDa. The antibody is not validated for WB since the results obtained are not reproducible at the moment.
Lane 1 Hek-BTLA (30ug)
Lane 2 Hek-Bcl7b (30ug)
Lane 3 Tonsil (100ug)
Lane 4 DOHH2 cell line (100ug)
Lane 5 Daudi cell line (100ug)
Lane 6 YT cell line (100ug)
Lane 7 Karpas 422 cell line (100ug)
Lane 8 REH cell line (100ug)
Lane 9 LP1 cell line (100ug)